Hotspot

trnR-CCG-rbcL

Genome · 25729-25961 · 233 bp

Back to Rhodobryum

Pi 0.0826

MarkerSeek score 75.40

Primer available yes

Label trnR-CCG-rbcL

Feature Metadata

label_name: trnR-CCG-rbcL
feature_type: igs
region: Genome
length_bp: 233
variable_sites: 18
pi: 0.0826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-CCG-rbcL_p1	ATCCACCAAATCGGCGTTTA	CGCCGGTACTGTAGTAGGTA	45.0	55.0	57.9	58.0	962-964 bp	1.0	83.3
trnR-CCG-rbcL_p2	TAATCCACCAAATCGGCGTT	CGCCGGTACTGTAGTAGGTA	45.0	55.0	57.9	58.0	964-966 bp	1.0	83.3
trnR-CCG-rbcL_p3	TCCACCAAATCGGCGTTTAT	CGCCGGTACTGTAGTAGGTA	45.0	55.0	57.9	58.0	961-963 bp	1.0	83.3
trnR-CCG-rbcL_p4	CTTCATCCATAGGCTGCCAG	CGCCGGTACTGTAGTAGGTA	55.0	55.0	58.1	58.0	1205-1207 bp	1.0	82.7
trnR-CCG-rbcL_p5	AGGTTCCGCGATCAATCAAA	CGCCGGTACTGTAGTAGGTA	45.0	55.0	57.9	58.0	1187 bp	0.5	58.8