Hotspot

infA-rpl36

Genome · 6457-6566 · 110 bp

Back to Rhodobryum

Pi 0.0935

MarkerSeek score 74.90

Primer available yes

Label infA-rpl36

Feature Metadata

label_name: infA-rpl36
feature_type: igs
region: Genome
length_bp: 110
variable_sites: 10
pi: 0.0935
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rpl36_p1	ATCGGGAAGCACGAGAAAAA	CCTTGATCGATCAAAACGCG	45.0	50.0	57.8	57.9	1004-1006 bp	1.0	80.9
infA-rpl36_p2	ATCGGGAAGCACGAGAAAAA	AAGCTGTATCTCGTCCTGGA	45.0	50.0	57.8	57.9	1060-1062 bp	1.0	80.3
infA-rpl36_p3	AAGACGGTTCAAGTACCAGC	CCTTGATCGATCAAAACGCG	50.0	50.0	57.8	57.9	1286 bp	0.5	58.1
infA-rpl36_p4	ACCGCCTTCGTAGTAAATCG	CCTTGATCGATCAAAACGCG	50.0	50.0	57.8	57.9	630 bp	0.5	57.8
infA-rpl36_p5	AAGACGGTTCAAGTACCAGC	AAGCTGTATCTCGTCCTGGA	50.0	50.0	57.8	57.9	1342 bp	0.5	57.5