primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpl23_p1	trnI-CAU-rpl23	1	TGAACGGTTAAAGCACCCAA	AAACGTTTGTGACCTCCTCC	20	20	45.0	50.0	57.9	58.0	0.0	13.8	0.091060	145	386	791-797	1.000000	83.8
trnI-CAU-rpl23_p2	trnI-CAU-rpl23	2	AACGGTTAAAGCACCCAACT	AAACGTTTGTGACCTCCTCC	20	20	45.0	50.0	57.9	58.0	0.0	13.8	0.098337	145	386	789-795	1.000000	83.8
trnI-CAU-rpl23_p3	trnI-CAU-rpl23	3	CCCAACTCATAATTGGCGGA	AAACGTTTGTGACCTCCTCC	20	20	50.0	50.0	57.9	58.0	0.0	13.8	0.088162	145	386	776-776	0.500000	59.3
trnI-CAU-rpl23_p4	trnI-CAU-rpl23	4	GATTCGCAGGTTCAACTCCT	AAACGTTTGTGACCTCCTCC	20	20	50.0	50.0	57.9	58.0	0.0	13.8	0.137433	145	386	758-758	0.500000	58.9
trnI-CAU-rpl23_p5	trnI-CAU-rpl23	5	CCCAACTCATAATTGGCGGA	TTTGTGACCTCCTCCTCTGT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.154488	145	386	771-771	0.500000	58.8
infA-rpl36_p1	infA-rpl36	1	ATCGGGAAGCACGAGAAAAA	CCTTGATCGATCAAAACGCG	20	20	45.0	50.0	57.8	57.9	0.0	33.8	0.256067	6457	6566	1004-1006	1.000000	80.9
infA-rpl36_p2	infA-rpl36	2	ATCGGGAAGCACGAGAAAAA	AAGCTGTATCTCGTCCTGGA	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.324309	6457	6566	1060-1062	1.000000	80.3
infA-rpl36_p3	infA-rpl36	3	AAGACGGTTCAAGTACCAGC	CCTTGATCGATCAAAACGCG	20	20	50.0	50.0	57.8	57.9	0.0	33.8	0.242474	6457	6566	1286-1286	0.500000	58.1
infA-rpl36_p4	infA-rpl36	4	ACCGCCTTCGTAGTAAATCG	CCTTGATCGATCAAAACGCG	20	20	50.0	50.0	57.8	57.9	0.0	33.8	0.276446	6457	6566	630-630	0.500000	57.8
infA-rpl36_p5	infA-rpl36	5	AAGACGGTTCAAGTACCAGC	AAGCTGTATCTCGTCCTGGA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.310716	6457	6566	1342-1342	0.500000	57.5
rps12-rpl20_p1	rps12-rpl20	1	CGGATGTCCTCAACGAAGAG	TGAGCTCCCTGAAATCCAGA	20	20	55.0	50.0	58.1	58.0	0.0	12.1	0.128498	14724	15659	1059-1108	1.000000	82.2
rps12-rpl20_p2	rps12-rpl20	2	ACAAAATCTCCAGCCCTTCG	TGAGCTCCCTGAAATCCAGA	20	20	50.0	50.0	58.2	58.0	0.0	12.1	0.216746	14724	15659	1079-1128	1.000000	81.5
rps12-rpl20_p3	rps12-rpl20	3	CTCAACGAAGAGGGGTTTGT	TGAGCTCCCTGAAATCCAGA	20	20	50.0	50.0	57.7	58.0	0.0	12.1	0.299249	14724	15659	1051-1100	1.000000	80.9
rps12-rpl20_p4	rps12-rpl20	4	CGGATGTCCTCAACGAAGAG	GCTCCCTGAAATCCAGATGT	20	20	55.0	50.0	58.1	57.3	0.0	0.0	0.810588	14724	15659	1056-1105	1.000000	76.8
rps12-rpl20_p5	rps12-rpl20	5	ACAAAATCTCCAGCCCTTCG	GCTCCCTGAAATCCAGATGT	20	20	50.0	50.0	58.2	57.3	0.0	0.0	0.898836	14724	15659	1076-1125	1.000000	76.0
trnR-CCG-rbcL_p1	trnR-CCG-rbcL	1	ATCCACCAAATCGGCGTTTA	CGCCGGTACTGTAGTAGGTA	20	20	45.0	55.0	57.9	58.0	6.8	1.9	0.163268	25729	25961	962-964	1.000000	83.3
trnR-CCG-rbcL_p2	trnR-CCG-rbcL	2	TAATCCACCAAATCGGCGTT	CGCCGGTACTGTAGTAGGTA	20	20	45.0	55.0	57.9	58.0	6.8	1.9	0.163268	25729	25961	964-966	1.000000	83.3
trnR-CCG-rbcL_p3	trnR-CCG-rbcL	3	TCCACCAAATCGGCGTTTAT	CGCCGGTACTGTAGTAGGTA	20	20	45.0	55.0	57.9	58.0	6.8	1.9	0.163268	25729	25961	961-963	1.000000	83.3
trnR-CCG-rbcL_p4	trnR-CCG-rbcL	4	CTTCATCCATAGGCTGCCAG	CGCCGGTACTGTAGTAGGTA	20	20	55.0	55.0	58.1	58.0	22.7	1.9	0.152897	25729	25961	1205-1207	1.000000	82.7
trnR-CCG-rbcL_p5	trnR-CCG-rbcL	5	AGGTTCCGCGATCAATCAAA	CGCCGGTACTGTAGTAGGTA	20	20	45.0	55.0	57.9	58.0	8.2	1.9	0.154391	25729	25961	1187-1187	0.500000	58.8
trnF-GAA-trnL-UAA_p1	trnF-GAA-trnL-UAA	1	TCCTCTGCTCTACCAACTGA	CGTTGGCTTTATTGACCGTG	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.846171	32939	32998	135-135	1.000000	75.6
trnF-GAA-trnL-UAA_p2	trnF-GAA-trnL-UAA	2	TCAGTCCTCTGCTCTACCAA	CGTTGGCTTTATTGACCGTG	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.846171	32939	32998	139-139	1.000000	75.5
trnF-GAA-trnL-UAA_p3	trnF-GAA-trnL-UAA	3	TTCAGTCCTCTGCTCTACCA	CGTTGGCTTTATTGACCGTG	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.846171	32939	32998	140-140	1.000000	75.5
trnF-GAA-trnL-UAA_p4	trnF-GAA-trnL-UAA	4	AGTCCTCTGCTCTACCAACT	CGTTGGCTTTATTGACCGTG	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.855394	32939	32998	137-137	1.000000	75.5
trnF-GAA-trnL-UAA_p5	trnF-GAA-trnL-UAA	5	GTCCTCTGCTCTACCAACTG	CGTTGGCTTTATTGACCGTG	20	20	55.0	50.0	57.3	57.8	0.0	0.0	0.905086	32939	32998	136-136	1.000000	75.1
psbZ-trnS-UGA_p1	psbZ-trnS-UGA	1	CCAACTGAAAGGCAATAGTCA	CATAAACCACTCGGCCATCT	21	20	42.9	50.0	56.2	57.7	0.0	2.2	3.176997	43057	43269	255-259	1.000000	62.4
psbZ-trnS-UGA_p2	psbZ-trnS-UGA	2	CCAACTGAAAGGCAATAGTCA	TAAACCACTCGGCCATCTCT	21	20	42.9	50.0	56.2	58.4	0.0	2.2	3.276430	43057	43269	253-257	1.000000	61.6
psbZ-trnS-UGA_p3	psbZ-trnS-UGA	3	CCAACTGAAAGGCAATAGTCA	TCAAGACCGGAGCCATAAAC	21	20	42.9	50.0	56.2	57.6	0.0	9.5	3.241524	43057	43269	268-272	1.000000	61.5
psbZ-trnS-UGA_p4	psbZ-trnS-UGA	4	CCAACTGAAAGGCAATAGTCA	GTTTTCAAGACCGGAGCCAT	21	20	42.9	50.0	56.2	58.5	0.0	9.5	3.310205	43057	43269	272-276	1.000000	60.8
psbZ-trnS-UGA_p5	psbZ-trnS-UGA	5	CCAACTGAAAGGCAATAGTCA	CAAGACCGGAGCCATAAACC	21	20	42.9	55.0	56.2	58.6	0.0	9.5	3.465407	43057	43269	267-271	1.000000	59.7
trnE-UUC-trnY-GUA_p1	trnE-UUC-trnY-GUA	1	CTCTCTTTCAAGGAGGCGAC	TTTGAACCAGCGTAGGCATT	20	20	55.0	45.0	58.0	58.1	0.0	10.5	0.098910	46939	47054	229-229	1.000000	82.0
trnE-UUC-trnY-GUA_p2	trnE-UUC-trnY-GUA	2	CTCTCTTTCAAGGAGGCGAC	TGGATTTGAACCAGCGTAGG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.111146	46939	47054	233-233	1.000000	81.9
trnE-UUC-trnY-GUA_p3	trnE-UUC-trnY-GUA	3	ATCGTCTAGTGGCCTAGGAC	TTTGAACCAGCGTAGGCATT	20	20	55.0	45.0	58.0	58.1	14.8	10.5	0.111647	46939	47054	251-251	1.000000	81.7
trnE-UUC-trnY-GUA_p4	trnE-UUC-trnY-GUA	4	CATCGTCTAGTGGCCTAGGA	TTTGAACCAGCGTAGGCATT	20	20	55.0	45.0	58.0	58.1	14.8	10.5	0.113053	46939	47054	252-252	1.000000	81.6
trnE-UUC-trnY-GUA_p5	trnE-UUC-trnY-GUA	5	ATCGTCTAGTGGCCTAGGAC	TGGATTTGAACCAGCGTAGG	20	20	55.0	50.0	58.0	57.9	14.8	0.0	0.123884	46939	47054	255-255	1.000000	81.5
ycf2_p1	ycf2	1	GGATCTGCTTCACCCCAAAA	GCGTCAGTCTCTCTCCAAAA	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.517183	53403	53522	612-612	1.000000	80.4
ycf2_p2	ycf2	2	AATTGGATCTGCTTCACCCC	GCGTCAGTCTCTCTCCAAAA	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.590512	53403	53522	616-616	1.000000	79.8
ycf2_p3	ycf2	3	GGATCTGCTTCACCCCAAAA	TTTACGCGTCAGTCTCTCTC	20	20	50.0	50.0	58.1	57.1	0.0	18.6	0.972887	53403	53522	617-617	1.000000	76.8
ycf2_p4	ycf2	4	GGATCTGCTTCACCCCAAAA	TTACGCGTCAGTCTCTCTCC	20	20	50.0	55.0	58.1	58.9	0.0	18.6	0.991554	53403	53522	616-616	1.000000	76.6
ycf2_p5	ycf2	5	AATTGGATCTGCTTCACCCC	TTTACGCGTCAGTCTCTCTC	20	20	50.0	50.0	57.9	57.1	0.0	18.6	1.046216	53403	53522	621-621	1.000000	76.1
ycf2-trnH-GUG_p1	ycf2-trnH-GUG	1	TTTTGGAGAGAGACTGACGC	GCCCGTAGTAGGTATCTGGT	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.448002	54384	54756	1233-1240	1.000000	80.5
ycf2-trnH-GUG_p2	ycf2-trnH-GUG	2	TTTTGGAGAGAGACTGACGC	TGGCCCGTAGTAGGTATCTG	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.449418	54384	54756	1235-1242	1.000000	80.5
ycf2-trnH-GUG_p3	ycf2-trnH-GUG	3	TTTTGGAGAGAGACTGACGC	TGTTGATAGTCAAGGCCGTG	20	20	50.0	50.0	57.6	57.9	0.0	13.1	0.533845	54384	54756	1149-1156	1.000000	79.9
ycf2-trnH-GUG_p4	ycf2-trnH-GUG	4	TTTTGGAGAGAGACTGACGC	GTTGATAGTCAAGGCCGTGT	20	20	50.0	50.0	57.6	57.9	0.0	18.6	0.535301	54384	54756	1148-1155	1.000000	79.9
ycf2-trnH-GUG_p5	ycf2-trnH-GUG	5	TTTTGGAGAGAGACTGACGC	GGTGTAGCTGGTGTATTCGG	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.504607	54384	54756	1466-1473	1.000000	79.7
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	AGTCGCACTTAAAAGCCGAG	GCGGCCAAAGAAGCTTTAAG	20	20	50.0	50.0	58.6	58.0	6.4	2.2	0.587925	58617	59292	748-761	1.000000	80.6
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	TAGTCGCACTTAAAAGCCGA	GCGGCCAAAGAAGCTTTAAG	20	20	45.0	50.0	57.3	58.0	3.0	2.2	0.746077	58617	59292	749-762	1.000000	79.3
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	TCGCACTTAAAAGCCGAGTA	GCGGCCAAAGAAGCTTTAAG	20	20	45.0	50.0	57.3	58.0	3.0	2.2	0.746077	58617	59292	746-759	1.000000	79.3
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	CGCACTTAAAAGCCGAGTAC	GCGGCCAAAGAAGCTTTAAG	20	20	50.0	50.0	57.2	58.0	0.0	2.2	0.799586	58617	59292	745-758	1.000000	78.9
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	ACCCTTGTCAATGGTTTGGA	GCGGCCAAAGAAGCTTTAAG	20	20	45.0	50.0	57.2	58.0	6.7	2.2	0.783077	58617	59292	805-818	1.000000	78.7
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	AATGATCCTGGCCGTAATCC	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	57.4	58.2	0.0	17.2	0.785551	62150	62262	168-171	1.000000	79.9
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	TCAAATGATCCTGGCCGTAA	GGGTTCGAATCCCTCTCTCT	20	20	45.0	55.0	56.9	58.2	0.0	17.2	1.308027	62150	62262	171-174	1.000000	75.6
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	AAATGATCCTGGCCGTAATCC	GGGTTCGAATCCCTCTCTCT	21	20	47.6	55.0	58.1	58.2	0.0	17.2	1.353120	62150	62262	169-172	1.000000	75.3
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	CTATCAAATGATCCTGGCCGT	GGGTTCGAATCCCTCTCTCT	21	20	47.6	55.0	57.9	58.2	0.0	17.2	1.348638	62150	62262	174-177	1.000000	75.1
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	ATCAAATGATCCTGGCCGTA	GGGTTCGAATCCCTCTCTCT	20	20	45.0	55.0	56.7	58.2	0.0	17.2	1.539791	62150	62262	172-175	1.000000	73.7
trnS-GCU-psaM_p1	trnS-GCU-psaM	1	TTAAACCACTCGGCCATCTC	TGTAGCTCTTGTCAGTGCTT	20	20	50.0	45.0	57.6	56.8	2.2	4.3	1.604928	62349	62628	361-373	1.000000	73.7
trnS-GCU-psaM_p2	trnS-GCU-psaM	2	TAAACCACTCGGCCATCTCT	TGTAGCTCTTGTCAGTGCTT	20	20	50.0	45.0	58.4	56.8	2.2	4.3	1.639834	62349	62628	360-372	1.000000	73.4
trnS-GCU-psaM_p3	trnS-GCU-psaM	3	TTAAACCACTCGGCCATCTC	TGTAGCTCTTGTCAGTGCTTT	20	21	50.0	42.9	57.6	57.5	2.2	4.3	1.872155	62349	62628	361-373	1.000000	71.6
trnS-GCU-psaM_p4	trnS-GCU-psaM	4	TTAAACCACTCGGCCATCTC	TTGTAGCTCTTGTCAGTGCTT	20	21	50.0	42.9	57.6	57.5	2.2	4.3	1.872155	62349	62628	362-374	1.000000	71.5
trnS-GCU-psaM_p5	trnS-GCU-psaM	5	TAAACCACTCGGCCATCTCT	TGTAGCTCTTGTCAGTGCTTT	20	21	50.0	42.9	58.4	57.5	2.2	4.3	1.907062	62349	62628	360-372	1.000000	71.3
trnG-GCC_p1	trnG-GCC	1	TATTCATCCGAGCCTAGCCA	GTCATCTCCTTCAGCTGTCG	20	20	50.0	55.0	58.0	58.1	0.0	14.7	0.089763	63224	63887	1936-1948	1.000000	82.6
trnG-GCC_p2	trnG-GCC	2	TATTCATCCGAGCCTAGCCA	AGAAGACCTCTGTCCTGTCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.078046	63224	63887	1502-1515	1.000000	82.5
trnG-GCC_p3	trnG-GCC	3	TATTCATCCGAGCCTAGCCA	TCTCCTTCAGCTGTCGAAGA	20	20	50.0	50.0	58.0	58.1	0.0	14.7	0.098624	63224	63887	1932-1944	1.000000	82.5
trnG-GCC_p4	trnG-GCC	4	TATTCATCCGAGCCTAGCCA	TCATCTCCTTCAGCTGTCGA	20	20	50.0	50.0	58.0	58.2	0.0	14.7	0.170258	63224	63887	1935-1947	1.000000	81.9
trnG-GCC_p5	trnG-GCC	5	TATTCATCCGAGCCTAGCCA	CCGGAAGCATTTGCACAATT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.092848	63224	63887	2034-2034	0.500000	59.3
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGACAGGACAGAGGTCTTCT	GCAGAAATTGTACGTTCTACCA	20	22	55.0	40.9	58.1	56.7	0.0	0.0	3.331328	64091	64238	311-312	1.000000	58.7
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGACAGGACAGAGGTCTTCT	TGCAGAAATTGTACGTTCTACC	20	22	55.0	40.9	58.1	56.7	0.0	0.0	3.331328	64091	64238	312-313	1.000000	58.7
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGACAGGACAGAGGTCTTCT	TGCAGAAATTGTACGTTCTACCA	20	23	55.0	39.1	58.1	58.4	0.0	0.0	3.435190	64091	64238	312-313	1.000000	57.9
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	ATGGACAGGACAGAGGTCTT	GCAGAAATTGTACGTTCTACCA	20	22	50.0	40.9	57.7	56.7	0.0	0.0	3.596969	64091	64238	313-314	1.000000	56.6
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	ATGGACAGGACAGAGGTCTT	TGCAGAAATTGTACGTTCTACC	20	22	50.0	40.9	57.7	56.7	0.0	0.0	3.596969	64091	64238	314-315	1.000000	56.6
rpoC1_p1	rpoC1	1	AGAAGAGCATTTTGGCTGGT	AGAATCAGACCATTCCGTCA	20	20	45.0	45.0	57.7	56.3	0.0	0.0	2.044438	73853	76620	2980-2980	0.500000	43.6
rpoC1_p2	rpoC1	2	AGAGCATTTTGGCTGGTTCT	AGAATCAGACCATTCCGTCA	20	20	45.0	45.0	57.7	56.3	0.0	0.0	2.044438	73853	76620	2977-2977	0.500000	43.6
rpoC1_p3	rpoC1	3	GAGCATTTTGGCTGGTTCTG	AGAATCAGACCATTCCGTCA	20	20	50.0	45.0	57.6	56.3	0.0	0.0	2.104051	73853	76620	2976-2976	0.500000	43.2
rpoC1_p4	rpoC1	4	AGAAGAGCATTTTGGCTGGT	AGAATCAGACCATTCCGTCAT	20	21	45.0	42.9	57.7	56.8	0.0	0.0	2.500702	73853	76620	2980-2980	0.500000	40.0
rpoC1_p5	rpoC1	5	AGAGCATTTTGGCTGGTTCT	AGAATCAGACCATTCCGTCAT	20	21	45.0	42.9	57.7	56.8	0.0	0.0	2.500702	73853	76620	2977-2977	0.500000	40.0
trnC-GCA-ycf66_p1	trnC-GCA-ycf66	1	AACTACAAAGGTGCCTTGGG	TGGCGTTTGGATCCAATTCT	20	20	50.0	45.0	58.0	57.8	9.9	27.5	0.219000	80200	80683	1421-1421	0.500000	58.2
trnC-GCA-ycf66_p2	trnC-GCA-ycf66	2	ATCATGACTCTTGCGACACC	TGGCGTTTGGATCCAATTCT	20	20	50.0	45.0	58.0	57.8	0.0	27.5	0.230112	80200	80683	1444-1444	0.500000	58.2
trnC-GCA-ycf66_p3	trnC-GCA-ycf66	3	AAGTGGTAAGGCAGAGGACT	TGGCGTTTGGATCCAATTCT	20	20	50.0	45.0	58.0	57.8	0.0	27.5	0.245871	80200	80683	801-801	0.500000	58.0
trnC-GCA-ycf66_p4	trnC-GCA-ycf66	4	GCGATATGGCCAAGTGGTAA	TGGCGTTTGGATCCAATTCT	20	20	50.0	45.0	58.0	57.8	11.0	27.5	0.252583	80200	80683	812-812	0.500000	58.0
trnC-GCA-ycf66_p5	trnC-GCA-ycf66	5	ATCCTTTACCCCCAGTTCGA	TGGCGTTTGGATCCAATTCT	20	20	50.0	45.0	58.0	57.8	0.0	27.5	0.253243	80200	80683	777-777	0.500000	58.0
ndhB_p1	ndhB	1	CTCGAACCTCCACGCTTTAA	GCACAAGGAAAAGCTCTTGC	20	20	50.0	50.0	57.9	58.2	0.0	11.7	0.298678	82384	84538	2743-2759	1.000000	81.1
ndhB_p2	ndhB	2	CCATTTCACCATCAAGGCCT	GCACAAGGAAAAGCTCTTGC	20	20	50.0	50.0	58.1	58.2	0.0	11.7	0.367502	82384	84538	2694-2710	1.000000	80.6
ndhB_p3	ndhB	3	CTCGAACCTCCACGCTTTAA	CAAGACGCTTAGGTGGATCC	20	20	50.0	55.0	57.9	58.1	0.0	4.2	0.133222	82384	84538	2809-2809	0.500000	58.9
ndhB_p4	ndhB	4	CCATTTCACCATCAAGGCCT	CAAGACGCTTAGGTGGATCC	20	20	50.0	55.0	58.1	58.1	0.0	4.2	0.202046	82384	84538	2760-2760	0.500000	58.4
ndhB_p5	ndhB	5	ACCATTTCACCATCAAGGCC	CAAGACGCTTAGGTGGATCC	20	20	50.0	55.0	58.4	58.1	0.0	4.2	0.498170	82384	84538	2761-2761	0.500000	56.0