Hotspot

ccsA-ndhD

SSC · 112520-112789 · 270 bp

Back to Rhodiola

Pi 0.0305

MarkerSeek score 66.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 270
variable_sites: 53
pi: 0.0305
unique_haplotype_count: 46
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAGGGATTCTTTCCGGAGCA	ACCCGGATTTCGTGTTTTCA	50.0	45.0	58.1	58.0	559-621 bp	1.0	90.8
ccsA-ndhD_p2	AATGAGGCCTGGGGATCATA	GCATATGGGTTGGTTCGGAT	50.0	50.0	57.9	58.0	1191-1253 bp	1.0	89.3
ccsA-ndhD_p3	TGAGGCCTGGGGATCATATT	GCATATGGGTTGGTTCGGAT	50.0	50.0	57.9	58.0	1189-1251 bp	1.0	89.3
ccsA-ndhD_p4	TAGGGATTCTTTCCGGAGCA	GCATATGGGTTGGTTCGGAT	50.0	50.0	58.1	58.0	1220-1282 bp	1.0	89.2
ccsA-ndhD_p5	TAGGGATTCTTTCCGGAGCA	CGTAATGGCAATGGGGATGA	50.0	50.0	58.1	58.0	732-794 bp	0.96	89.2