Hotspot

ndhF-rpl32

SSC · 110178-110758 · 581 bp

Back to Rhodiola

Pi 0.0177

MarkerSeek score 74.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 581
variable_sites: 71
pi: 0.0177
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAAGGAGAGGT	TTAACGCTGCCCAATATCCC	55.0	50.0	58.3	58.0	716-751 bp	0.98	88.2
ndhF-rpl32_p2	CTGGAAGTGGAAGGAGAGGT	AAAAGGCTTTTAACGCTGCC	55.0	45.0	58.3	57.9	725-760 bp	0.98	87.1
ndhF-rpl32_p3	TTTTTGTTGCCGTCGGAAAA	TTAACGCTGCCCAATATCCC	40.0	50.0	57.4	58.0	767-802 bp	1.0	86.6
ndhF-rpl32_p4	CTGGAAGTGGAAGGAGAGGT	TACGCTTTTTGGCCAGAGAA	55.0	45.0	58.3	57.7	671-706 bp	0.98	86.3
ndhF-rpl32_p5	TTTTGTTGCCGTCGGAAAAA	TTAACGCTGCCCAATATCCC	40.0	50.0	57.4	58.0	766-801 bp	0.98	85.5