Hotspot

psbE-petL

LSC · 62978-64036 · 1059 bp

Back to Rhodiola

Pi 0.0141

MarkerSeek score 82.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1059
variable_sites: 124
pi: 0.0141
unique_haplotype_count: 49
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.8	58.0	1154-1181 bp	1.0	90.4
psbE-petL_p2	CAACCCGCAATGAATAGGGA	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.9	58.0	1231-1258 bp	0.98	90.4
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	CGTATCTTGCTCAGCCCAAT	55.0	50.0	58.2	58.0	1149-1176 bp	1.0	90.1
psbE-petL_p4	ACCAACCCGCAATGAATAGG	CGTATCTTGCTCAGCCCAAT	50.0	50.0	58.2	58.0	1233-1260 bp	0.98	88.9
psbE-petL_p5	CAACCCGCAATGAATAGGGA	AAGTCGTATCTTGCTCAGCC	50.0	50.0	57.9	57.7	1235-1262 bp	0.96	87.4