Hotspot

petA-psbJ

LSC · 61192-62200 · 1009 bp

Back to Rhodiola

Pi 0.0151

MarkerSeek score 72.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1009
variable_sites: 118
pi: 0.0151
unique_haplotype_count: 49
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCACAAATCTTTTTGGTTCT	ACTGGTATTGCTGTGATCGG	36.4	50.0	57.2	57.7	489-1199 bp	1.0	67.5
petA-psbJ_p2	TGGCACAAATCTTTTTGGTTCT	CTGGTATTGCTGTGATCGGT	36.4	50.0	57.2	57.7	488-1198 bp	1.0	67.5
petA-psbJ_p3	TGGCACAAATCTTTTTGGTTCTT	ACTGGTATTGCTGTGATCGG	34.8	50.0	57.9	57.7	489-1199 bp	1.0	64.7
petA-psbJ_p4	TGGCACAAATCTTTTTGGTTCTT	CTGGTATTGCTGTGATCGGT	34.8	50.0	57.9	57.7	488-1198 bp	1.0	64.7
petA-psbJ_p5	TGGCACAAATCTTTTTGGTTCT	TGGTATTGCTGTGATCGGTT	36.4	45.0	57.2	57.2	487-1197 bp	1.0	63.7