Hotspot

ycf4-cemA

LSC · 58721-59320 · 600 bp

Back to Rhodiola

Pi 0.00959

MarkerSeek score 68.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 600
variable_sites: 54
pi: 0.00959
unique_haplotype_count: 45
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGACTCCACGCGAAATTGAA	AAAGAGAGATCAACCAGGGT	45.0	45.0	58.1	55.7	741-779 bp	1.0	71.9
ycf4-cemA_p2	TTGACTCCACGCGAAATTGA	AAAGAGAGATCAACCAGGGT	45.0	45.0	58.1	55.7	742-780 bp	1.0	71.9
ycf4-cemA_p3	ACAAAAAGCTGCGGAATTGG	AAAGAGAGATCAACCAGGGT	45.0	45.0	57.8	55.7	722-760 bp	1.0	71.9
ycf4-cemA_p4	AGAATTTGACTCCACGCGAA	AAAGAGAGATCAACCAGGGT	45.0	45.0	57.8	55.7	747-785 bp	1.0	71.7
ycf4-cemA_p5	GACTCCACGCGAAATTGAAC	AAAGAGAGATCAACCAGGGT	50.0	45.0	57.8	55.7	740-778 bp	0.98	69.9