Pi
0.0117
MarkerSeek score
72.60
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GACCACCGCATTACAAATGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 464-491 bp | 1.0 | 90.5 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 446-473 bp | 1.0 | 90.4 |
| trnT-UGU-trnL-UAA_p3 | GACCACCGCATTACAAATGC | GTTGCGTCTACCAATTTCGC | 50.0 | 50.0 | 58.1 | 58.1 | 473-500 bp | 1.0 | 89.4 |
| trnT-UGU-trnL-UAA_p4 | GACCACCGCATTACAAATGC | CGTTGCGTCTACCAATTTCG | 50.0 | 50.0 | 58.1 | 57.9 | 474-501 bp | 1.0 | 89.2 |
| trnT-UGU-trnL-UAA_p5 | TCCATAAAAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 497-524 bp | 0.98 | 88.6 |