Hotspot

lhbA-trnG-UCC

LSC · 35626-36220 · 595 bp

Back to Rhodiola

Pi 0.011

MarkerSeek score 75.10

Primer available yes

Label lhbA-trnG-UCC

Feature Metadata

label_name: lhbA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 51
pi: 0.011
unique_haplotype_count: 45
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lhbA-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	698-764 bp	1.0	87.0
lhbA-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	696-762 bp	1.0	86.7
lhbA-trnG-UCC_p3	TTGCTTCTCCTGATGGTTGG	AAATCCATTGGGCGGATAGC	50.0	50.0	57.8	58.4	732-798 bp	1.0	85.2
lhbA-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	AAATCCATTGGGCGGATAGC	55.0	50.0	58.3	58.4	730-796 bp	1.0	84.8
lhbA-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	TACCATTCGACCATATCCGC	50.0	50.0	57.8	57.3	671-737 bp	1.0	82.8