Hotspot

trnT-GGU-psbD

LSC · 31739-32426 · 688 bp

Back to Rhodiola

Pi 0.0116

MarkerSeek score 66.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 62
pi: 0.0116
unique_haplotype_count: 47
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.3	53.0	659-746 bp	1.0	50.8
trnT-GGU-psbD_p2	GGCCCTTTTAACTCAGTGGT	TTTACCAAGGGCTATAGTCA	50.0	40.0	57.7	53.0	697-784 bp	1.0	50.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	55.0	40.0	58.1	53.0	682-769 bp	1.0	50.7
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTATAGTCAT	55.0	40.0	58.1	52.8	681-768 bp	1.0	50.7
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.1	53.0	685-772 bp	0.98	49.6