Hotspot

trnD-GUC-trnY-GUA

LSC · 30309-30608 · 300 bp

Back to Rhodiola

Pi 0.0251

MarkerSeek score 64.70

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 300
variable_sites: 35
pi: 0.0251
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTGGATACGTCGGGACTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.2	57.8	416-442 bp	0.94	84.7
trnD-GUC-trnY-GUA_p2	ATTGGATACGTCGGGACTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.2	57.8	415-441 bp	0.94	84.7
trnD-GUC-trnY-GUA_p3	ATTGGATACGTCGGGACTGA	AAGTGATTGTTCCTGGGTCG	50.0	50.0	58.2	57.8	474-500 bp	0.94	84.0
trnD-GUC-trnY-GUA_p4	ATTGGATACGTCGGGACTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.2	58.4	437-463 bp	0.94	83.3
trnD-GUC-trnY-GUA_p5	ATTGGATACGTCGGGACTGA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.2	57.6	436-462 bp	0.94	83.1