Hotspot

trnC-GCA-petN

LSC · 27182-28015 · 834 bp

Back to Rhodiola

Pi 0.0137

MarkerSeek score 71.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 99
pi: 0.0137
unique_haplotype_count: 46
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	543-934 bp	1.0	91.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	537-928 bp	1.0	89.8
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	561-952 bp	1.0	89.1
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TTAGAGTCCACTTCGTCCCC	50.0	55.0	58.1	58.4	599-990 bp	1.0	87.8
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	555-946 bp	1.0	87.6