Hotspot

rps16-trnQ-UUG

LSC · 5865-6562 · 698 bp

Back to Rhodiola

Pi 0.0113

MarkerSeek score 67.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 62
pi: 0.0113
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	769-1593 bp	1.0	85.8
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	780-1604 bp	1.0	85.3
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTATGCGGAGGTTCGAATCC	45.0	55.0	58.1	58.2	776-1600 bp	1.0	84.7
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTATGCGGAGGTTCGAATCC	50.0	55.0	58.2	58.2	787-1611 bp	1.0	84.2
rps16-trnQ-UUG_p5	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	790-1614 bp	1.0	83.8