Hotspot

trnH-GUG-psbA

LSC · 73-324 · 252 bp

Back to Rhodiola

Pi 0.0426

MarkerSeek score 74.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 72
pi: 0.0426
unique_haplotype_count: 46
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	227-341 bp	1.0	90.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	241-355 bp	1.0	90.4
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	240-354 bp	1.0	90.4
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	230-344 bp	1.0	90.4
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	242-356 bp	0.98	89.6