Hotspot

ndhA

SSC · 129779-131973 · 2195 bp

Back to Rhizophora

Pi 0.00364

MarkerSeek score 59.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2195
variable_sites: 12
pi: 0.00364
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGGCCAGTTACCATAGGGAG	ATGGGGGAAGTTGATCGTTG	55.0	50.0	58.2	57.9	2329-2332 bp	1.0	77.9
ndhA_p2	GAGGCCAGTTACCATAGGGA	ATGGGGGAAGTTGATCGTTG	55.0	50.0	58.2	57.9	2330-2333 bp	1.0	77.8
ndhA_p3	AGGCCAGTTACCATAGGGAG	GGGGGAAGTTGATCGTTGAA	55.0	50.0	58.2	57.8	2327-2330 bp	1.0	77.4
ndhA_p4	GAGGCCAGTTACCATAGGGA	GGGGGAAGTTGATCGTTGAA	55.0	50.0	58.2	57.8	2328-2331 bp	1.0	77.3
ndhA_p5	TGAGGCCAGTTACCATAGGG	ATGGGGGAAGTTGATCGTTG	55.0	50.0	58.5	57.9	2331-2334 bp	1.0	75.5