Hotspot

rps15-ycf1

SSC · 133529-134055 · 527 bp

Back to Rhizophora

Pi 0.00266

MarkerSeek score 61.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 527
variable_sites: 2
pi: 0.00266
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTGAAATTCGACGGATCCCC	ACGCTAGTCGGTTCAGTATG	50.0	50.0	58.0	57.2	627-652 bp	1.0	76.4
rps15-ycf1_p2	TTGAAATTCGACGGATCCCC	CGCTAGTCGGTTCAGTATGT	50.0	50.0	58.0	57.2	626-651 bp	1.0	76.4
rps15-ycf1_p3	CCCCTGTTTTCGTCTTGTGA	ACGCTAGTCGGTTCAGTATG	50.0	50.0	58.0	57.2	611-636 bp	1.0	76.4
rps15-ycf1_p4	CCCCTGTTTTCGTCTTGTGA	CGCTAGTCGGTTCAGTATGT	50.0	50.0	58.0	57.2	610-635 bp	1.0	76.4
rps15-ycf1_p5	TCCCCTGTTTTCGTCTTGTG	CGCTAGTCGGTTCAGTATGT	50.0	50.0	58.0	57.2	611-636 bp	1.0	76.4