Hotspot

trnL-TAG-ccsA

SSC · 124165-124298 · 134 bp

Back to Rhizophora

Pi 0.0308
MarkerSeek score 62.20
Primer available yes
Label trnL-TAG-ccsA

Feature Metadata

label_name
trnL-TAG-ccsA
feature_type
igs
region
SSC
length_bp
134
variable_sites
6
pi
0.0308
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-TAG-ccsA_p1 GTGAAATCGGTAGACACGCT ACAATTGAAACGACCGAAAAAGA 50.0 34.8 58.0 57.8 263-267 bp 1.0 57.1
trnL-TAG-ccsA_p2 TGAAATCGGTAGACACGCTG ACAATTGAAACGACCGAAAAAGA 50.0 34.8 58.0 57.8 262-266 bp 1.0 57.1
trnL-TAG-ccsA_p3 TAGACACGCTGCTCTTAGGA ACAATTGAAACGACCGAAAAAGA 50.0 34.8 57.9 57.8 253-257 bp 1.0 56.2
trnL-TAG-ccsA_p4 GTAGACACGCTGCTCTTAGG ACAATTGAAACGACCGAAAAAGA 55.0 34.8 57.8 57.8 254-258 bp 1.0 55.7
trnL-TAG-ccsA_p5 GGTAGACACGCTGCTCTTAG ACAATTGAAACGACCGAAAAAGA 55.0 34.8 57.8 57.8 255-259 bp 1.0 55.6