Hotspot

psbZ-trnG-GCC

LSC · 40919-42004 · 1086 bp

Back to Rhizophora

Pi 0.0465

MarkerSeek score 90.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1086
variable_sites: 74
pi: 0.0465
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 90.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	1240-1482 bp	1.0	84.2
psbZ-trnG-GCC_p2	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	1236-1478 bp	1.0	82.6
psbZ-trnG-GCC_p3	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	1239-1481 bp	1.0	81.5
psbZ-trnG-GCC_p4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.8	57.8	1238-1480 bp	1.0	77.8
psbZ-trnG-GCC_p5	CTTCTCCTGATGGTTGGTCG	CGGATAGCGGGAATCGAAC	55.0	57.9	58.0	58.2	1262-1504 bp	1.0	76.0