Hotspot

trnM-CAU-psbD

LSC · 35149-37509 · 2361 bp

Back to Rhizophora

Pi 0.00734

MarkerSeek score 58.80

Primer available yes

Label trnM-CAU-psbD

Feature Metadata

label_name: trnM-CAU-psbD
feature_type: igs
region: LSC
length_bp: 2361
variable_sites: 24
pi: 0.00734
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-psbD_p1	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.3	58.0	2502-2544 bp	1.0	82.4
trnM-CAU-psbD_p2	GGCGTAAGTCATCGGTTCAA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.3	58.0	2504-2546 bp	1.0	82.2
trnM-CAU-psbD_p3	CGCTATGGTAAGGCGTAAGT	GGCCAGACCAACCTACAAAA	50.0	50.0	57.5	58.0	2513-2555 bp	1.0	80.6
trnM-CAU-psbD_p4	CGCTATGGTAAGGCGTAAGT	TAGGCCAGACCAACCTACAA	50.0	50.0	57.5	58.0	2515-2557 bp	1.0	80.5
trnM-CAU-psbD_p5	GGCGTAAGTCATCGGTTCAA	ATAGGCCAGACCAACCTACA	50.0	50.0	58.3	57.7	2505-2547 bp	1.0	80.4