Hotspot

trnE-UUC-trnT-GGU

LSC · 33787-35076 · 1290 bp

Back to Rhizophora

Pi 0.0348

MarkerSeek score 77.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1290
variable_sites: 51
pi: 0.0348
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	1108-1393 bp	1.0	85.2
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	1087-1372 bp	1.0	85.1
trnE-UUC-trnT-GGU_p3	TCGTAATACCCTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	57.6	58.0	1144-1429 bp	1.0	84.1
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	1105-1390 bp	1.0	84.1
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	1084-1369 bp	1.0	84.0