Hotspot

trnC-GCA-petN

LSC · 29933-31178 · 1246 bp

Back to Rhizophora

Pi 0.0405

MarkerSeek score 85.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1246
variable_sites: 65
pi: 0.0405
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCAGTTCAAATCCGGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1139-1301 bp	1.0	86.5
trnC-GCA-petN_p2	TTTCCCAGTTCAAATCCGGG	TGCCCCATACTACGAGTGAA	50.0	50.0	58.1	58.1	1188-1350 bp	1.0	86.4
trnC-GCA-petN_p3	TTTCCCAGTTCAAATCCGGG	ACTTCTGCCCCATACTACGA	50.0	50.0	58.1	57.8	1193-1355 bp	1.0	86.3
trnC-GCA-petN_p4	TTTCCCAGTTCAAATCCGGG	TTCTGCCCCATACTACGAGT	50.0	50.0	58.1	57.8	1191-1353 bp	1.0	86.3
trnC-GCA-petN_p5	TTTCCCAGTTCAAATCCGGG	CACTTCTGCCCCATACTACG	50.0	55.0	58.1	57.8	1194-1356 bp	1.0	85.7