Hotspot

atpF-atpH

LSC · 14072-14721 · 650 bp

Back to Rhizophora

Pi 0.00522

MarkerSeek score 63.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 5
pi: 0.00522
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCCAATGACCCAAAGAAACG	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.9	58.0	737-789 bp	1.0	81.3
atpF-atpH_p2	ATTAAGCCCGAAACTCCCAG	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.6	58.0	763-815 bp	1.0	78.4
atpF-atpH_p3	GGCCAATGACCCAAAGAAAC	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.5	58.0	738-790 bp	1.0	78.2
atpF-atpH_p4	GCCAATGACCCAAAGAAACG	CTGGTTGTGGCATTAGCACT	50.0	50.0	57.9	58.5	738-790 bp	1.0	77.7
atpF-atpH_p5	GCCAATGACCCAAAGAAACG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	806-858 bp	1.0	75.7