Hotspot

clpP

Genome · 7643-8275 · 633 bp

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Pi 0.132

MarkerSeek score 81.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 633
variable_sites: 82
pi: 0.132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCTCCCTCCCCATGGAAAAT	ACCCGCTACAGTTCCCTTAT	50.0	50.0	58.0	58.1	1205 bp	1.0	89.8
clpP_p2	TCTCCCTCCCCATGGAAAAT	ATACCCGCTACAGTTCCCTT	50.0	50.0	58.0	58.1	1207 bp	1.0	89.7
clpP_p3	TCTCCCTCCCCATGGAAAAT	CCAAATCTAAGACGGGACCG	50.0	55.0	58.0	58.1	1798 bp	0.5	59.5
clpP_p4	TCCGACTATTCTCATGGCCT	CCAAATCTAAGACGGGACCG	50.0	55.0	57.9	58.1	1689 bp	0.5	58.8
clpP_p5	GACTATTCTCATGGCCTGGC	CCAAATCTAAGACGGGACCG	55.0	55.0	58.1	58.1	1686 bp	0.5	58.6