Hotspot

accD

Genome · 54586-57657 · 3072 bp

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Pi 0.199

MarkerSeek score 78.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 3072
variable_sites: 545
pi: 0.199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GAAGGCCCTAAAGACTCTGC	TAATTTGTCGACGTCGGTCC	55.0	50.0	58.0	58.0	292 bp	0.5	59.6
accD_p2	TGCGGAACACAGACAAGAAA	TAATTTGTCGACGTCGGTCC	45.0	50.0	58.0	58.0	249 bp	0.5	59.6
accD_p3	TGTCAACAGAGACGGCAAAA	TAATTTGTCGACGTCGGTCC	45.0	50.0	58.0	58.0	369 bp	0.5	59.6
accD_p4	GAAGGCCCTAAAGACTCTGC	ATATAACGGCCCATTTGCGT	55.0	45.0	58.0	58.0	422 bp	0.5	59.6
accD_p5	TGCGGAACACAGACAAGAAA	ATATAACGGCCCATTTGCGT	45.0	45.0	58.0	58.0	379 bp	0.5	59.6