Hotspot

rrn16-trnV-GAC

Genome · 109719-109995 · 277 bp

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Pi 0.097

MarkerSeek score 73.60

Primer available yes

Label rrn16-trnV-GAC

Feature Metadata

label_name: rrn16-trnV-GAC
feature_type: igs
region: Genome
length_bp: 277
variable_sites: 23
pi: 0.097
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnV-GAC_p1	CCTCAGCCTACGGGGTATTA	ATACGAAAAGAAGTCCCGGC	55.0	50.0	58.0	58.0	641-701 bp	1.0	87.3
rrn16-trnV-GAC_p2	CCTCAGCCTACGGGGTATTA	GCTCCAAGTTGGTCAAGACA	55.0	50.0	58.0	58.0	623-683 bp	1.0	87.1
rrn16-trnV-GAC_p3	TAACGCTTGCATCCTCTGTC	ATACGAAAAGAAGTCCCGGC	50.0	50.0	58.0	58.0	969-1029 bp	1.0	85.4
rrn16-trnV-GAC_p4	CCTCAGCCTACGGGGTATTA	ATGAATCGTCCTTTCCACGG	55.0	50.0	58.0	58.0	1186-1248 bp	1.0	85.2
rrn16-trnV-GAC_p5	TAACGCTTGCATCCTCTGTC	ATGAATCGTCCTTTCCACGG	50.0	50.0	58.0	58.0	1514-1576 bp	1.0	84.0