Hotspot

trnI-GAU-rrn16

Genome · 107916-108221 · 306 bp

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Pi 0.137

MarkerSeek score 79.90

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: Genome
length_bp: 306
variable_sites: 37
pi: 0.137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	TGGGCACATTGAACTATCCG	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.0	57.7	445-1215 bp	1.0	84.3
trnI-GAU-rrn16_p2	GGGCACATTGAACTATCCGT	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.0	57.7	444-1214 bp	1.0	84.3
trnI-GAU-rrn16_p3	CTATCCGTGTGGTTGAGAGC	CAAGGTAGCCGTACTGGAAG	55.0	55.0	58.1	57.7	432-1202 bp	1.0	84.1
trnI-GAU-rrn16_p4	TGGGCACATTGAACTATCCG	GAAGGTGCGACTGGATTACC	50.0	55.0	58.0	58.3	429-1199 bp	1.0	84.0
trnI-GAU-rrn16_p5	TGGGCACATTGAACTATCCG	GGAAGGTGCGACTGGATTAC	50.0	55.0	58.0	58.3	430-1200 bp	1.0	84.0