Hotspot

trnA-UGC-trnI-GAU

Genome · 106832-106891 · 60 bp

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Pi 0.0909

MarkerSeek score 74.30

Primer available yes

Label trnA-UGC-trnI-GAU

Feature Metadata

label_name: trnA-UGC-trnI-GAU
feature_type: igs
region: Genome
length_bp: 60
variable_sites: 5
pi: 0.0909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-trnI-GAU_p1	CAACGACCCAATTGCAAGAG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	157-162 bp	1.0	82.8
trnA-UGC-trnI-GAU_p2	CAACGACCCAATTGCAAGAG	GCGAGGTCTCTGGTTCAAAT	50.0	50.0	57.7	57.9	142-147 bp	1.0	82.6
trnA-UGC-trnI-GAU_p3	CCGCCTGGACAATTAGACAT	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	191-196 bp	1.0	81.9
trnA-UGC-trnI-GAU_p4	CCGCCTGGACAATTAGACAT	GCGAGGTCTCTGGTTCAAAT	50.0	50.0	57.7	57.9	176-181 bp	1.0	81.5
trnA-UGC-trnI-GAU_p5	AGACATCCAACCCGTAATCG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.4	58.0	177-182 bp	1.0	80.1