Hotspot

trnM-CAU-accD

Genome · 54025-54585 · 561 bp

Back to Rhipsalis

Pi 0.318

MarkerSeek score 82.00

Primer available yes

Label trnM-CAU-accD

Feature Metadata

label_name: trnM-CAU-accD
feature_type: igs
region: Genome
length_bp: 561
variable_sites: 163
pi: 0.318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-accD_p1	TACGCCTTAACGACCAATGG	TAATTTGTCGACGTCGGTCC	50.0	50.0	58.0	58.0	1651 bp	0.5	59.7
trnM-CAU-accD_p2	TACGCCTTAACGACCAATGG	ATATAACGGCCCATTTGCGT	50.0	45.0	58.0	58.0	1781 bp	0.5	59.7
trnM-CAU-accD_p3	TACGCCTTAACGACCAATGG	GCAGAGTCTTTAGGGCCTTC	50.0	55.0	58.0	58.0	1379 bp	0.5	59.6
trnM-CAU-accD_p4	TACGCCTTAACGACCAATGG	TTTCTTGTCTGTGTTCCGCA	50.0	45.0	58.0	58.0	1422 bp	0.5	59.6
trnM-CAU-accD_p5	TACGCCTTAACGACCAATGG	TTTTGCCGTCTCTGTTGACA	50.0	45.0	58.0	58.0	1302 bp	0.5	59.6