primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5471	5730	333-485	1.000000	81.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5471	5730	344-496	1.000000	81.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	5471	5730	363-515	1.000000	80.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.375253	5471	5730	374-526	1.000000	80.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GTAAGGCATCGGGTTTTGGT	20	20	45.0	50.0	58.1	58.5	8.1	0.0	0.597945	5471	5730	364-516	1.000000	78.3
trnS-GCU-clpP_p1	trnS-GCU-clpP	1	ACGGAAAGAGAGGGATTCGA	TATCCTAGCGTGAGGGAGTG	20	20	50.0	55.0	58.2	58.0	0.0	5.1	0.168688	7219	7642	853-891	1.000000	88.7
trnS-GCU-clpP_p2	trnS-GCU-clpP	2	ACGGAAAGAGAGGGATTCGA	ATTCTCCTCCGACCAAGACA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.212764	7219	7642	818-856	1.000000	88.5
trnS-GCU-clpP_p3	trnS-GCU-clpP	3	ACGGAAAGAGAGGGATTCGA	TTCTCCTCCGACCAAGACAT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.212764	7219	7642	817-855	1.000000	88.5
trnS-GCU-clpP_p4	trnS-GCU-clpP	4	ACGGAAAGAGAGGGATTCGA	CTTGTTAGATCACGCCACGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.160549	7219	7642	596-596	0.500000	58.7
trnS-GCU-clpP_p5	trnS-GCU-clpP	5	ACGGAAAGAGAGGGATTCGA	TTGTTAGATCACGCCACGAG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.160549	7219	7642	595-595	0.500000	58.7
clpP_p1	clpP	1	TCTCCCTCCCCATGGAAAAT	ACCCGCTACAGTTCCCTTAT	20	20	50.0	50.0	58.0	58.1	15.2	0.0	0.124802	7643	8275	1205-1205	1.000000	89.8
clpP_p2	clpP	2	TCTCCCTCCCCATGGAAAAT	ATACCCGCTACAGTTCCCTT	20	20	50.0	50.0	58.0	58.1	15.2	0.0	0.124802	7643	8275	1207-1207	1.000000	89.7
clpP_p3	clpP	3	TCTCCCTCCCCATGGAAAAT	CCAAATCTAAGACGGGACCG	20	20	50.0	55.0	58.0	58.1	15.2	9.6	0.064357	7643	8275	1798-1798	0.500000	59.5
clpP_p4	clpP	4	TCCGACTATTCTCATGGCCT	CCAAATCTAAGACGGGACCG	20	20	50.0	55.0	57.9	58.1	3.3	9.6	0.152172	7643	8275	1689-1689	0.500000	58.8
clpP_p5	clpP	5	GACTATTCTCATGGCCTGGC	CCAAATCTAAGACGGGACCG	20	20	55.0	55.0	58.1	58.1	11.1	9.6	0.174523	7643	8275	1686-1686	0.500000	58.6
rpoC1-rpoB_p1	rpoC1-rpoB	1	TGGCCCAAGCACTTATTTGT	TCCAGATTAATAGGATGGAAGCT	20	23	45.0	39.1	58.0	56.6	0.0	5.3	4.449300	21825	21853	128-128	1.000000	46.3
rpoC1-rpoB_p2	rpoC1-rpoB	2	TGTGGCCCAAGCACTTATTT	TCCAGATTAATAGGATGGAAGCT	20	23	45.0	39.1	58.0	56.6	0.0	5.3	4.449300	21825	21853	130-130	1.000000	46.3
rpoC1-rpoB_p3	rpoC1-rpoB	3	TTGTGGCCCAAGCACTTATT	TCCAGATTAATAGGATGGAAGCT	20	23	45.0	39.1	58.0	56.6	0.0	5.3	4.449300	21825	21853	131-131	1.000000	46.2
rpoC1-rpoB_p4	rpoC1-rpoB	4	TTTGTGGCCCAAGCACTTAT	TCCAGATTAATAGGATGGAAGCT	20	23	45.0	39.1	58.0	56.6	0.0	5.3	4.449300	21825	21853	132-132	1.000000	46.2
rpoC1-rpoB_p5	rpoC1-rpoB	5	TTTTGTGGCCCAAGCACTTA	TCCAGATTAATAGGATGGAAGCT	20	23	45.0	39.1	58.2	56.6	0.0	5.3	4.659271	21825	21853	133-133	1.000000	44.5
petN-psbM_p1	petN-psbM	1	ATTGTAAGTCTCGCTTGGGC	AAAACGGTTAGCCAAAATGA	20	20	50.0	35.0	58.3	54.0	0.0	0.0	4.280046	26615	27290	656-784	1.000000	57.8
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	AAAACGGTTAGCCAAAATGA	20	20	50.0	35.0	57.6	54.0	0.0	0.0	4.369975	26615	27290	594-722	1.000000	57.0
petN-psbM_p3	petN-psbM	3	ATTGTAAGTCTCGCTTGGGC	AAACGGTTAGCCAAAATGAT	20	20	50.0	35.0	58.3	53.7	0.0	0.0	4.521337	26615	27290	655-783	1.000000	55.8
petN-psbM_p4	petN-psbM	4	ATTGTAAGTCTCGCTTGGGC	AACGGTTAGCCAAAATGATT	20	20	50.0	35.0	58.3	53.7	0.0	0.0	4.521337	26615	27290	654-782	1.000000	55.8
petN-psbM_p5	petN-psbM	5	ATGGGGAAGAAGTGGACTCT	AACGGTTAGCCAAAATGATT	20	20	50.0	35.0	57.6	53.7	0.0	0.0	4.611266	26615	27290	592-720	1.000000	55.1
trnM-CAU-accD_p1	trnM-CAU-accD	1	TACGCCTTAACGACCAATGG	TAATTTGTCGACGTCGGTCC	20	20	50.0	50.0	58.0	58.0	0.0	35.0	0.031683	54025	54585	1651-1651	0.500000	59.7
trnM-CAU-accD_p2	trnM-CAU-accD	2	TACGCCTTAACGACCAATGG	ATATAACGGCCCATTTGCGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.037277	54025	54585	1781-1781	0.500000	59.7
trnM-CAU-accD_p3	trnM-CAU-accD	3	TACGCCTTAACGACCAATGG	GCAGAGTCTTTAGGGCCTTC	20	20	50.0	55.0	58.0	58.0	0.0	13.5	0.044195	54025	54585	1379-1379	0.500000	59.6
trnM-CAU-accD_p4	trnM-CAU-accD	4	TACGCCTTAACGACCAATGG	TTTCTTGTCTGTGTTCCGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044195	54025	54585	1422-1422	0.500000	59.6
trnM-CAU-accD_p5	trnM-CAU-accD	5	TACGCCTTAACGACCAATGG	TTTTGCCGTCTCTGTTGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044195	54025	54585	1302-1302	0.500000	59.6
accD_p1	accD	1	GAAGGCCCTAAAGACTCTGC	TAATTTGTCGACGTCGGTCC	20	20	55.0	50.0	58.0	58.0	0.0	35.0	0.050005	54878	54997	292-292	0.500000	59.6
accD_p2	accD	2	TGCGGAACACAGACAAGAAA	TAATTTGTCGACGTCGGTCC	20	20	45.0	50.0	58.0	58.0	0.0	35.0	0.050005	54878	54997	249-249	0.500000	59.6
accD_p3	accD	3	TGTCAACAGAGACGGCAAAA	TAATTTGTCGACGTCGGTCC	20	20	45.0	50.0	58.0	58.0	0.0	35.0	0.050005	54878	54997	369-369	0.500000	59.6
accD_p4	accD	4	GAAGGCCCTAAAGACTCTGC	ATATAACGGCCCATTTGCGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.055599	54878	54997	422-422	0.500000	59.6
accD_p5	accD	5	TGCGGAACACAGACAAGAAA	ATATAACGGCCCATTTGCGT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.055599	54878	54997	379-379	0.500000	59.6
ycf1_p1	ycf1	1	GTCGTATTGGAGCCTGGATC	ACAGAACACCTAGGTCGACA	20	20	55.0	50.0	57.8	58.0	2.6	18.9	0.165222	93673	93792	642-642	0.500000	58.7
ycf1_p2	ycf1	2	GTCGTATTGGAGCCTGGATC	TCCTCCTTCTCCACCCAATT	20	20	55.0	50.0	57.8	57.9	2.6	0.0	0.216099	93673	93792	610-610	0.500000	58.3
ycf1_p3	ycf1	3	GTCGTATTGGAGCCTGGATC	CGCCCTTTTTCTTGTTGCAA	20	20	55.0	45.0	57.8	58.1	2.6	0.0	0.223097	93673	93792	670-670	0.500000	58.2
ycf1_p4	ycf1	4	GTCGTATTGGAGCCTGGATC	ACCCTTTTCGCCCTTTTTCT	20	20	55.0	45.0	57.8	57.9	2.6	0.0	0.238483	93673	93792	678-678	0.500000	58.1
ycf1_p5	ycf1	5	GTCGTATTGGAGCCTGGATC	CTCCGTTTTGCAGCAATGTT	20	20	55.0	45.0	57.8	57.9	2.6	9.1	0.294939	93673	93792	771-771	0.500000	57.6
trnA-UGC-trnI-GAU_p1	trnA-UGC-trnI-GAU	1	CAACGACCCAATTGCAAGAG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.7	58.0	4.9	0.0	0.341137	106832	106891	157-162	1.000000	82.8
trnA-UGC-trnI-GAU_p2	trnA-UGC-trnI-GAU	2	CAACGACCCAATTGCAAGAG	GCGAGGTCTCTGGTTCAAAT	20	20	50.0	50.0	57.7	57.9	4.9	0.0	0.437159	106832	106891	142-147	1.000000	82.6
trnA-UGC-trnI-GAU_p3	trnA-UGC-trnI-GAU	3	CCGCCTGGACAATTAGACAT	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.336646	106832	106891	191-196	1.000000	81.9
trnA-UGC-trnI-GAU_p4	trnA-UGC-trnI-GAU	4	CCGCCTGGACAATTAGACAT	GCGAGGTCTCTGGTTCAAAT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.432668	106832	106891	176-181	1.000000	81.5
trnA-UGC-trnI-GAU_p5	trnA-UGC-trnI-GAU	5	AGACATCCAACCCGTAATCG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.601486	106832	106891	177-182	1.000000	80.1
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	TGGGCACATTGAACTATCCG	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.0	57.7	0.0	7.8	0.332210	107916	108221	445-1215	1.000000	84.3
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	GGGCACATTGAACTATCCGT	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.0	57.7	0.0	7.8	0.333640	107916	108221	444-1214	1.000000	84.3
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	CTATCCGTGTGGTTGAGAGC	CAAGGTAGCCGTACTGGAAG	20	20	55.0	55.0	58.1	57.7	0.0	7.8	0.367358	107916	108221	432-1202	1.000000	84.1
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	TGGGCACATTGAACTATCCG	GAAGGTGCGACTGGATTACC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.383806	107916	108221	429-1199	1.000000	84.0
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	TGGGCACATTGAACTATCCG	GGAAGGTGCGACTGGATTAC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.383806	107916	108221	430-1200	1.000000	84.0
rrn16-trnV-GAC_p1	rrn16-trnV-GAC	1	CCTCAGCCTACGGGGTATTA	ATACGAAAAGAAGTCCCGGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025318	109719	109995	641-701	1.000000	87.3
rrn16-trnV-GAC_p2	rrn16-trnV-GAC	2	CCTCAGCCTACGGGGTATTA	GCTCCAAGTTGGTCAAGACA	20	20	55.0	50.0	58.0	58.0	0.0	21.4	0.038052	109719	109995	623-683	1.000000	87.1
rrn16-trnV-GAC_p3	rrn16-trnV-GAC	3	TAACGCTTGCATCCTCTGTC	ATACGAAAAGAAGTCCCGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030093	109719	109995	969-1029	1.000000	85.4
rrn16-trnV-GAC_p4	rrn16-trnV-GAC	4	CCTCAGCCTACGGGGTATTA	ATGAATCGTCCTTTCCACGG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.029546	109719	109995	1186-1248	1.000000	85.2
rrn16-trnV-GAC_p5	rrn16-trnV-GAC	5	TAACGCTTGCATCCTCTGTC	ATGAATCGTCCTTTCCACGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034321	109719	109995	1514-1576	1.000000	84.0
rps7-trnL-CAA_p1	rps7-trnL-CAA	1	CTTTGGCACATTTTCGTCGT	GTCTCGCGTGTCTACCATTT	20	20	45.0	50.0	57.6	58.0	0.0	5.8	0.407226	112671	115153	2407-2538	1.000000	88.7
rps7-trnL-CAA_p2	rps7-trnL-CAA	2	CTTTGGCACATTTTCGTCGT	CTCGCGTGTCTACCATTTCA	20	20	45.0	50.0	57.6	58.0	0.0	5.8	0.408597	112671	115153	2405-2536	1.000000	88.7
rps7-trnL-CAA_p3	rps7-trnL-CAA	3	AGCTTTGGCACATTTTCGTC	GTCTCGCGTGTCTACCATTT	20	20	45.0	50.0	57.6	58.0	0.0	5.8	0.430106	112671	115153	2409-2540	1.000000	88.6
rps7-trnL-CAA_p4	rps7-trnL-CAA	4	GAGCTTTGGCACATTTTCGT	GTCTCGCGTGTCTACCATTT	20	20	45.0	50.0	57.6	58.0	0.0	5.8	0.430106	112671	115153	2410-2541	1.000000	88.6
rps7-trnL-CAA_p5	rps7-trnL-CAA	5	AGCTTTGGCACATTTTCGTC	CTCGCGTGTCTACCATTTCA	20	20	45.0	50.0	57.6	58.0	0.0	5.8	0.431477	112671	115153	2407-2538	1.000000	88.5
trnI-CAU-trnH-GUG_p1	trnI-CAU-trnH-GUG	1	GAATGGTTAAAGCGCCCAAC	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	2.3	0.2	0.138121	121860	122055	331-331	0.500000	58.9
trnI-CAU-trnH-GUG_p2	trnI-CAU-trnH-GUG	2	GAATGGTTAAAGCGCCCAAC	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	2.3	0.0	0.142482	121860	122055	317-317	0.500000	58.9
trnI-CAU-trnH-GUG_p3	trnI-CAU-trnH-GUG	3	GAATGGTTAAAGCGCCCAAC	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	2.3	0.0	0.143990	121860	122055	316-316	0.500000	58.8
trnI-CAU-trnH-GUG_p4	trnI-CAU-trnH-GUG	4	GAATGGTTAAAGCGCCCAAC	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	2.3	0.0	0.143990	121860	122055	318-318	0.500000	58.8
trnI-CAU-trnH-GUG_p5	trnI-CAU-trnH-GUG	5	GCTAAGCATCCATGGCTGAA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.3	58.1	19.8	0.2	0.444977	121860	122055	348-348	0.500000	56.4