Hotspot

ycf3

LSC · 42189-44110 · 1922 bp

Back to Rhinanthus

Pi 0.00104

MarkerSeek score 58.70

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1922
variable_sites: 3
pi: 0.00104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGGTGCGTCCAAACCAAAAA	TAGAATGGAGGTGGCTAGCT	45.0	50.0	59.1	57.5	2043-2047 bp	1.0	67.5
ycf3_p2	TGGTGCGTCCAAACCAAAAA	GATCATTTTCCCGCGAGAGG	45.0	55.0	59.1	58.8	2078-2082 bp	1.0	65.1
ycf3_p3	TGGTGCGTCCAAACCAAAAA	GGATCATTTTCCCGCGAGAG	45.0	55.0	59.1	58.8	2079-2083 bp	1.0	65.1
ycf3_p4	TGGTGCGTCCAAACCAAAA	TAGAATGGAGGTGGCTAGCT	47.4	50.0	58.4	57.5	2043-2047 bp	1.0	65.0
ycf3_p5	TGGTGCGTCCAAACCAAAAA	GCGAGAGGGAATGAGAAGAA	45.0	50.0	59.1	57.0	2066-2070 bp	1.0	63.6