Hotspot

rps16

LSC · 5247-6320 · 1074 bp

Back to Rhinanthus

Pi 0.00124

MarkerSeek score 59.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1074
variable_sites: 2
pi: 0.00124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACAGCAGAAAATAAAGGGGG	AATTCCACACCGGTTCCAAA	45.0	45.0	55.6	57.9	1208 bp	1.0	60.2
rps16_p2	ACAGCAGAAAATAAAGGGGG	TTCCACACCGGTTCCAAATT	45.0	45.0	55.6	57.9	1206 bp	1.0	60.2
rps16_p3	ACAGCAGAAAATAAAGGGGG	ATCAATTCCACACCGGTTCC	45.0	50.0	55.6	58.2	1211 bp	1.0	59.5
rps16_p4	ACAGCAGAAAATAAAGGGGG	ATTCCACACCGGTTCCAAAT	45.0	45.0	55.6	57.7	1207 bp	1.0	58.4
rps16_p5	ACAGCAGAAAATAAAGGGGG	CGACAGCCGAATGAATGACT	45.0	50.0	55.6	58.4	1251 bp	1.0	58.0