Hotspot

rpoC1

LSC · 20948-23752 · 2805 bp

Back to Rhinanthus

Pi 0.000475
MarkerSeek score 44.60
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2805
variable_sites
2
pi
0.000475
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 CGGAATTCCTACTGGTATCGT AGGAAGGAAGCTTAATCGGA 47.6 45.0 57.0 55.9 2884 bp 1.0 47.5
rpoC1_p2 AGAGATCGGAATTCCTACTGGT AGGAAGGAAGCTTAATCGGA 45.5 45.0 58.1 55.9 2890 bp 1.0 46.8
rpoC1_p3 CGGAATTCCTACTGGTATCGT AGGAAGGAAGCTTAATCGGAA 47.6 42.9 57.0 56.7 2884 bp 1.0 45.8
rpoC1_p4 TCGGAATTCCTACTGGTATCG AGGAAGGAAGCTTAATCGGA 47.6 45.0 56.7 55.9 2885 bp 1.0 45.3
rpoC1_p5 AGAGATCGGAATTCCTACTGGT AGGAAGGAAGCTTAATCGGAA 45.5 42.9 58.1 56.7 2890 bp 1.0 45.1