Hotspot

rpl16

LSC · 80318-81590 · 1273 bp

Back to Rhinanthus

Pi 0.00183

MarkerSeek score 49.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1273
variable_sites: 3
pi: 0.00183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCCAAGAACTGTTTATGTTGGT	ACAATTTGATTCACCTTTGGA	39.1	33.3	58.2	53.9	1327-1328 bp	1.0	40.2
rpl16_p2	CCCAAGAACTGTTTATGTTGGT	ACAATTTGATTCACCTTTGGA	40.9	33.3	56.8	53.9	1326-1327 bp	1.0	40.2
rpl16_p3	TGTTTCATCCCAAGAACTGT	ACAATTTGATTCACCTTTGGA	40.0	33.3	54.8	53.9	1334-1335 bp	1.0	40.2
rpl16_p4	TCCATGTTTTTGTTTCATCCCA	ACAATTTGATTCACCTTTGGA	36.4	33.3	56.7	53.9	1344-1345 bp	1.0	40.2
rpl16_p5	TCCCAAGAACTGTTTATGTTGGT	AACAATTTGATTCACCTTTGGA	39.1	31.8	58.2	54.7	1328-1329 bp	1.0	40.2