Hotspot

psbA

LSC · 569-1627 · 1059 bp

Back to Rhinanthus

Pi 0.00126

MarkerSeek score 59.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1059
variable_sites: 2
pi: 0.00126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TAAAGGAGCAATAACCCCGC	ATTCGTTTGCTTGGGAGTCC	50.0	50.0	58.0	58.5	1210 bp	1.0	76.0
psbA_p2	TAACCCCGCTTGATAGACCA	ATTCGTTTGCTTGGGAGTCC	50.0	50.0	58.1	58.5	1199 bp	1.0	75.4
psbA_p3	GAGCAATAACCCCGCTTGAT	ATTCGTTTGCTTGGGAGTCC	50.0	50.0	58.3	58.5	1205 bp	1.0	73.9
psbA_p4	TAAAGGAGCAATAACCCCGC	TTTGCTTGGGAGTCCCTGAT	50.0	50.0	58.0	58.9	1205 bp	1.0	72.4
psbA_p5	TAACCCCGCTTGATAGACCA	TTTGCTTGGGAGTCCCTGAT	50.0	50.0	58.1	58.9	1194 bp	1.0	71.7