Hotspot

petD-rpoA

LSC · 76980-77335 · 356 bp

Back to Rhinanthus

Pi 0.00749

MarkerSeek score 31.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 4
pi: 0.00749
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TAACATAGGCTTGGCGGTTC	GGCGCTTTATTCTGTCTCCA	50.0	50.0	58.0	58.0	1412 bp	1.0	79.8
petD-rpoA_p2	TAACATAGGCTTGGCGGTTC	TGGGCGCTTTATTCTGTCTC	50.0	50.0	58.0	58.0	1414 bp	1.0	79.8
petD-rpoA_p3	ATGTAACATAGGCTTGGCGG	GGCGCTTTATTCTGTCTCCA	50.0	50.0	58.0	58.0	1415 bp	1.0	79.8
petD-rpoA_p4	ATGTAACATAGGCTTGGCGG	TGGGCGCTTTATTCTGTCTC	50.0	50.0	58.0	58.0	1417 bp	1.0	79.8
petD-rpoA_p5	TAACATAGGCTTGGCGGTTC	TTGTGTCAGAGGTCCTGGAT	50.0	50.0	58.0	58.0	1177 bp	1.0	79.8