Hotspot

psbB-psbT

LSC · 73277-73459 · 183 bp

Back to Rhinanthus

Pi 0

MarkerSeek score 29.60

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 183
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	AACTCGGGGATCCAACTACA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	58.1	57.6	311-314 bp	1.0	68.4
psbB-psbT_p2	ACTCGGGGATCCAACTACAA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	58.1	57.6	310-313 bp	1.0	68.4
psbB-psbT_p3	CCAAAAACTCGGGGATCCAA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	58.1	57.6	316-319 bp	1.0	68.2
psbB-psbT_p4	TTCCAAAAACTCGGGGATCC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.8	57.6	318-321 bp	1.0	67.2
psbB-psbT_p5	TCGGGGATCCAACTACAAGA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.8	57.6	308-311 bp	1.0	66.9