Hotspot

trnT-UGU-trnL-UAA

LSC · 46228-46962 · 735 bp

Back to Rhinanthus

Pi 0.00181

MarkerSeek score 59.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 2
pi: 0.00181
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	797 bp	1.0	77.2
trnT-UGU-trnL-UAA_p2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	848 bp	1.0	76.3
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	829 bp	1.0	76.3
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	791 bp	1.0	75.9
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	829 bp	1.0	75.1