Hotspot

trnH-GUG-psbA

LSC · 78-568 · 491 bp

Back to Rhinanthus

Pi 0.00272

MarkerSeek score 44.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 2
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTGGCTGCTAT	55.0	55.0	58.1	58.0	555-557 bp	1.0	79.2
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTGGCTGCTAT	50.0	55.0	57.9	58.0	569-571 bp	1.0	79.1
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTGGCTGCTAT	50.0	55.0	57.9	58.0	568-570 bp	1.0	79.1
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTGGCTGCTAT	50.0	55.0	57.9	58.0	570-572 bp	1.0	79.1
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	610-612 bp	1.0	77.8