primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTGGCTGCTAT	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.137162	78	568	555-557	1.000000	79.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTGGCTGCTAT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.141524	78	568	569-571	1.000000	79.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTGGCTGCTAT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.143031	78	568	568-570	1.000000	79.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTGGCTGCTAT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.143031	78	568	570-572	1.000000	79.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.301771	78	568	610-612	1.000000	77.8
psbA_p1	psbA	1	TAAAGGAGCAATAACCCCGC	ATTCGTTTGCTTGGGAGTCC	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.512273	569	1627	1210-1210	1.000000	76.0
psbA_p2	psbA	2	TAACCCCGCTTGATAGACCA	ATTCGTTTGCTTGGGAGTCC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.596559	569	1627	1199-1199	1.000000	75.4
psbA_p3	psbA	3	GAGCAATAACCCCGCTTGAT	ATTCGTTTGCTTGGGAGTCC	20	20	50.0	50.0	58.3	58.5	0.0	0.0	0.784934	569	1627	1205-1205	1.000000	73.9
psbA_p4	psbA	4	TAAAGGAGCAATAACCCCGC	TTTGCTTGGGAGTCCCTGAT	20	20	50.0	50.0	58.0	58.9	0.0	28.7	0.971401	569	1627	1205-1205	1.000000	72.4
psbA_p5	psbA	5	TAACCCCGCTTGATAGACCA	TTTGCTTGGGAGTCCCTGAT	20	20	50.0	50.0	58.1	58.9	0.0	28.7	1.055686	569	1627	1194-1194	1.000000	71.7
rps16_p1	rps16	1	ACAGCAGAAAATAAAGGGGG	AATTCCACACCGGTTCCAAA	20	20	45.0	45.0	55.6	57.9	0.0	3.0	2.488889	5247	6320	1208-1208	1.000000	60.2
rps16_p2	rps16	2	ACAGCAGAAAATAAAGGGGG	TTCCACACCGGTTCCAAATT	20	20	45.0	45.0	55.6	57.9	0.0	3.0	2.488889	5247	6320	1206-1206	1.000000	60.2
rps16_p3	rps16	3	ACAGCAGAAAATAAAGGGGG	ATCAATTCCACACCGGTTCC	20	20	45.0	50.0	55.6	58.2	0.0	3.0	2.579972	5247	6320	1211-1211	1.000000	59.5
rps16_p4	rps16	4	ACAGCAGAAAATAAAGGGGG	ATTCCACACCGGTTCCAAAT	20	20	45.0	45.0	55.6	57.7	0.0	3.0	2.716748	5247	6320	1207-1207	1.000000	58.4
rps16_p5	rps16	5	ACAGCAGAAAATAAAGGGGG	CGACAGCCGAATGAATGACT	20	20	45.0	50.0	55.6	58.4	0.0	0.0	2.769126	5247	6320	1251-1251	1.000000	58.0
rpoC1_p1	rpoC1	1	CGGAATTCCTACTGGTATCGT	AGGAAGGAAGCTTAATCGGA	21	20	47.6	45.0	57.0	55.9	22.1	0.0	4.072185	20948	23752	2884-2884	1.000000	47.5
rpoC1_p2	rpoC1	2	AGAGATCGGAATTCCTACTGGT	AGGAAGGAAGCTTAATCGGA	22	20	45.5	45.0	58.1	55.9	22.1	0.0	4.150926	20948	23752	2890-2890	1.000000	46.8
rpoC1_p3	rpoC1	3	CGGAATTCCTACTGGTATCGT	AGGAAGGAAGCTTAATCGGAA	21	21	47.6	42.9	57.0	56.7	22.1	0.0	4.287096	20948	23752	2884-2884	1.000000	45.8
rpoC1_p4	rpoC1	4	TCGGAATTCCTACTGGTATCG	AGGAAGGAAGCTTAATCGGA	21	20	47.6	45.0	56.7	55.9	22.1	0.0	4.346506	20948	23752	2885-2885	1.000000	45.3
rpoC1_p5	rpoC1	5	AGAGATCGGAATTCCTACTGGT	AGGAAGGAAGCTTAATCGGAA	22	21	45.5	42.9	58.1	56.7	22.1	0.0	4.365837	20948	23752	2890-2890	1.000000	45.1
ycf3_p1	ycf3	1	TGGTGCGTCCAAACCAAAAA	TAGAATGGAGGTGGCTAGCT	20	20	45.0	50.0	59.1	57.5	0.0	15.7	1.583342	42189	44110	2043-2047	1.000000	67.5
ycf3_p2	ycf3	2	TGGTGCGTCCAAACCAAAAA	GATCATTTTCCCGCGAGAGG	20	20	45.0	55.0	59.1	58.8	0.0	8.2	1.883056	42189	44110	2078-2082	1.000000	65.1
ycf3_p3	ycf3	3	TGGTGCGTCCAAACCAAAAA	GGATCATTTTCCCGCGAGAG	20	20	45.0	55.0	59.1	58.8	0.0	8.2	1.883056	42189	44110	2079-2083	1.000000	65.1
ycf3_p4	ycf3	4	TGGTGCGTCCAAACCAAAA	TAGAATGGAGGTGGCTAGCT	19	20	47.4	50.0	58.4	57.5	0.0	15.7	1.898994	42189	44110	2043-2047	1.000000	65.0
ycf3_p5	ycf3	5	TGGTGCGTCCAAACCAAAAA	GCGAGAGGGAATGAGAAGAA	20	20	45.0	50.0	59.1	57.0	0.0	0.0	2.074098	42189	44110	2066-2070	1.000000	63.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46228	46962	797-797	1.000000	77.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.482748	46228	46962	848-848	1.000000	76.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46228	46962	829-829	1.000000	76.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46228	46962	791-791	1.000000	75.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	46228	46962	829-829	1.000000	75.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	AAATTGTAACCAAGCGTCGC	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	57.7	57.8	9.8	0.0	0.522792	49965	51182	1267-1275	1.000000	76.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	AAATTGTAACCAAGCGTCGC	GAAGGTCTACGGTTCGAGTC	20	20	45.0	55.0	57.7	57.5	9.8	0.0	0.866565	49965	51182	1266-1274	1.000000	73.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AAATTGTAACCAAGCGTCGC	GTTCGAGTCCGTATAGCCCT	20	20	45.0	55.0	57.7	58.7	9.8	0.0	1.008859	49965	51182	1255-1263	1.000000	72.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	AAATTGTAACCAAGCGTCGC	AAGGTCTACGGTTCGAGTCC	20	20	45.0	55.0	57.7	58.8	9.8	0.0	1.153334	49965	51182	1265-1273	1.000000	71.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	AAATTGTAACCAAGCGTCGC	GAGAAGGTCTACGGTTCGAG	20	20	45.0	55.0	57.7	57.2	9.8	0.0	1.154384	49965	51182	1268-1276	1.000000	71.4
atpB-rbcL_p1	atpB-rbcL	1	GACCGATGATTTGGACCACA	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.409444	54204	54963	896-896	1.000000	77.0
atpB-rbcL_p2	atpB-rbcL	2	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.457837	54204	54963	921-921	1.000000	76.6
atpB-rbcL_p3	atpB-rbcL	3	ACCGATGATTTGGACCACAC	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	58.2	58.3	0.0	0.0	0.480278	54204	54963	895-895	1.000000	76.4
atpB-rbcL_p4	atpB-rbcL	4	TGGAAACCCCAGGATCAGAA	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	58.2	58.3	0.0	0.0	0.547591	54204	54963	851-851	1.000000	75.9
atpB-rbcL_p5	atpB-rbcL	5	CATCTTACCTGGCGGAAAGG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.3	58.3	0.0	0.0	0.633075	54204	54963	931-931	1.000000	75.2
accD-psaI_p1	accD-psaI	1	TCGTACCGCGCAATCTTTTA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	8.7	26.9	0.033461	58442	59072	790-790	1.000000	79.9
accD-psaI_p2	accD-psaI	2	CCAGAAGGGCTTATTCGACC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	0.0	26.9	0.072076	58442	59072	813-813	1.000000	79.6
accD-psaI_p3	accD-psaI	3	TCGTACCGCGCAATCTTTTA	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	58.0	57.9	8.7	26.8	0.109995	58442	59072	796-796	1.000000	79.3
accD-psaI_p4	accD-psaI	4	CCAGAAGGGCTTATTCGACC	AAGAAGCCATTGCAATTGCC	20	20	55.0	45.0	58.0	57.9	0.0	26.8	0.148610	58442	59072	819-819	1.000000	79.0
accD-psaI_p5	accD-psaI	5	TAATCGTACCGCGCAATCTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.8	58.0	8.7	26.9	0.239872	58442	59072	793-793	1.000000	78.3
clpP1_p1	clpP1	1	ACGGGTTTCAACAAAATATATT	ACGTTTCCACATCAAAGTGA	22	20	27.3	40.0	52.3	55.5	0.0	0.0	10.143537	69385	71330	2020-2021	1.000000	40.2
clpP1_p2	clpP1	2	ACGGGTTTCAACAAAATATATTT	ACGTTTCCACATCAAAGTGA	23	20	26.1	40.0	53.2	55.5	4.8	0.0	10.273428	69385	71330	2020-2021	1.000000	40.2
clpP1_p3	clpP1	3	ACGGGTTTCAACAAAATATATT	ACGTTTCCACATCAAAGTGAA	22	21	27.3	38.1	52.3	56.3	0.0	0.0	10.354691	69385	71330	2020-2021	1.000000	40.2
clpP1_p4	clpP1	4	ACGGGTTTCAACAAAATATATTTT	ACGTTTCCACATCAAAGTGA	24	20	25.0	40.0	54.0	55.5	16.1	0.0	10.475843	69385	71330	2020-2021	1.000000	40.2
clpP1_p5	clpP1	5	ACGGGTTTCAACAAAATATATTT	ACGTTTCCACATCAAAGTGAA	23	21	26.1	38.1	53.2	56.3	4.8	0.0	10.484583	69385	71330	2020-2021	1.000000	40.2
psbB_p1	psbB	1	CCGAAGAAGGAGAGTCCAGA	GCGAATGATACCAAAGCAGC	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.270304	71750	73276	1645-1655	1.000000	78.5
psbB_p2	psbB	2	TCCGAAGAAGGAGAGTCCAG	GCGAATGATACCAAAGCAGC	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.270304	71750	73276	1646-1656	1.000000	78.5
psbB_p3	psbB	3	CCGAAGAAGGAGAGTCCAGA	AATGATACCAAAGCAGCGGT	20	20	55.0	45.0	58.2	57.9	0.0	0.0	0.288034	71750	73276	1642-1652	1.000000	78.3
psbB_p4	psbB	4	TCCGAAGAAGGAGAGTCCAG	AATGATACCAAAGCAGCGGT	20	20	55.0	45.0	58.2	57.9	0.0	0.0	0.288034	71750	73276	1643-1653	1.000000	78.3
psbB_p5	psbB	5	TTCCGAAGAAGGAGAGTCCA	GCGAATGATACCAAAGCAGC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.415262	71750	73276	1647-1657	1.000000	77.3
psbB-psbT_p1	psbB-psbT	1	AACTCGGGGATCCAACTACA	TGGAACTTTAGGTGGTTCTCG	20	21	50.0	47.6	58.1	57.6	15.2	0.0	1.449276	73277	73459	311-314	1.000000	68.4
psbB-psbT_p2	psbB-psbT	2	ACTCGGGGATCCAACTACAA	TGGAACTTTAGGTGGTTCTCG	20	21	50.0	47.6	58.1	57.6	15.2	0.0	1.449276	73277	73459	310-313	1.000000	68.4
psbB-psbT_p3	psbB-psbT	3	CCAAAAACTCGGGGATCCAA	TGGAACTTTAGGTGGTTCTCG	20	21	50.0	47.6	58.1	57.6	15.2	0.0	1.478388	73277	73459	316-319	1.000000	68.2
psbB-psbT_p4	psbB-psbT	4	TTCCAAAAACTCGGGGATCC	TGGAACTTTAGGTGGTTCTCG	20	21	50.0	47.6	57.8	57.6	1.9	0.0	1.605950	73277	73459	318-321	1.000000	67.2
psbB-psbT_p5	psbB-psbT	5	TCGGGGATCCAACTACAAGA	TGGAACTTTAGGTGGTTCTCG	20	21	50.0	47.6	57.8	57.6	15.2	0.0	1.637541	73277	73459	308-311	1.000000	66.9
psbT-psbN_p1	psbT-psbN	1	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	21	20	47.6	55.0	57.6	58.4	0.0	0.0	1.824342	73568	73628	127-127	1.000000	67.3
psbT-psbN_p2	psbT-psbN	2	CGAGAACCACCTAAAGTTCCA	AGATCCATTCGAGGAACACG	21	20	47.6	50.0	57.6	57.4	0.0	0.0	1.982519	73568	73628	128-128	1.000000	66.0
psbT-psbN_p3	psbT-psbN	3	TCGAGAACCACCTAAAGTTCC	GATCCATTCGAGGAACACGG	21	20	47.6	55.0	57.3	58.4	0.0	0.0	2.100848	73568	73628	128-128	1.000000	65.1
psbT-psbN_p4	psbT-psbN	4	TCGAGAACCACCTAAAGTTCC	AGATCCATTCGAGGAACACG	21	20	47.6	50.0	57.3	57.4	0.0	0.0	2.259025	73568	73628	129-129	1.000000	63.8
psbT-psbN_p5	psbT-psbN	5	CGAGAACCACCTAAAGTTCCA	CGAGATCCATTCGAGGAACA	21	20	47.6	50.0	57.6	57.1	0.0	0.0	2.267442	73568	73628	130-130	1.000000	63.7
petD-rpoA_p1	petD-rpoA	1	TAACATAGGCTTGGCGGTTC	GGCGCTTTATTCTGTCTCCA	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.056856	76980	77335	1412-1412	1.000000	79.8
petD-rpoA_p2	petD-rpoA	2	TAACATAGGCTTGGCGGTTC	TGGGCGCTTTATTCTGTCTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.056856	76980	77335	1414-1414	1.000000	79.8
petD-rpoA_p3	petD-rpoA	3	ATGTAACATAGGCTTGGCGG	GGCGCTTTATTCTGTCTCCA	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.056857	76980	77335	1415-1415	1.000000	79.8
petD-rpoA_p4	petD-rpoA	4	ATGTAACATAGGCTTGGCGG	TGGGCGCTTTATTCTGTCTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.056857	76980	77335	1417-1417	1.000000	79.8
petD-rpoA_p5	petD-rpoA	5	TAACATAGGCTTGGCGGTTC	TTGTGTCAGAGGTCCTGGAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062708	76980	77335	1177-1177	1.000000	79.8
rpl16_p1	rpl16	1	TCCCAAGAACTGTTTATGTTGGT	ACAATTTGATTCACCTTTGGA	23	21	39.1	33.3	58.2	53.9	16.8	2.8	8.340316	80318	81590	1327-1328	1.000000	40.2
rpl16_p2	rpl16	2	CCCAAGAACTGTTTATGTTGGT	ACAATTTGATTCACCTTTGGA	22	21	40.9	33.3	56.8	53.9	16.8	2.8	8.341222	80318	81590	1326-1327	1.000000	40.2
rpl16_p3	rpl16	3	TGTTTCATCCCAAGAACTGT	ACAATTTGATTCACCTTTGGA	20	21	40.0	33.3	54.8	53.9	0.0	2.8	8.367166	80318	81590	1334-1335	1.000000	40.2
rpl16_p4	rpl16	4	TCCATGTTTTTGTTTCATCCCA	ACAATTTGATTCACCTTTGGA	22	21	36.4	33.3	56.7	53.9	0.0	2.8	8.443664	80318	81590	1344-1345	1.000000	40.2
rpl16_p5	rpl16	5	TCCCAAGAACTGTTTATGTTGGT	AACAATTTGATTCACCTTTGGA	23	22	39.1	31.8	58.2	54.7	16.8	2.8	8.496868	80318	81590	1328-1329	1.000000	40.2
ycf1_p1	ycf1	1	ACAGATGTCGTTTCTAGTCT	TTTCAGTACTCCCATGTTGG	20	20	40.0	45.0	53.5	55.0	0.0	0.0	7.574708	107062	108726	1807-1839	1.000000	40.4
ycf1_p2	ycf1	2	ACAGATGTCGTTTCTAGTCT	TTTTCAGTACTCCCATGTTGG	20	21	40.0	42.9	53.5	55.8	0.0	0.0	7.751260	107062	108726	1808-1840	1.000000	40.4
ycf1_p3	ycf1	3	ACAGATGTCGTTTCTAGTCT	TTTTTCAGTACTCCCATGTTGG	20	22	40.0	40.9	53.5	56.5	0.0	0.0	8.002288	107062	108726	1809-1841	1.000000	40.4
ycf1_p4	ycf1	4	AGAAATAAGGGAGGTTTGTG	TTTCAGTACTCCCATGTTGG	20	20	40.0	45.0	52.7	55.0	0.0	0.0	8.292115	107062	108726	1707-1739	1.000000	40.4
ycf1_p5	ycf1	5	ACAGATGTCGTTTCTAGTCTATCT	TTTCAGTACTCCCATGTTGG	24	20	37.5	45.0	56.7	55.0	0.0	0.0	8.296255	107062	108726	1807-1839	1.000000	40.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGTAGGGGGTATTGGACAG	CTCGCACTGCTTCCTAAGAG	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.272063	109759	110519	911-911	1.000000	78.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGTAGGGGGTATTGGACAG	CTCTCGCACTGCTTCCTAAG	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.272063	109759	110519	913-913	1.000000	78.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGTAGGGGGTATTGGACAG	TCCTAAGAGCAGCGTGTCTA	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.316258	109759	110519	900-900	1.000000	77.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGTAGGGGGTATTGGACAG	AGCAGCGTGTCTACCAATTT	20	20	55.0	45.0	57.8	57.8	0.0	0.0	0.376985	109759	110519	893-893	1.000000	77.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGTAGGGGGTATTGGACAG	CTAAGAGCAGCGTGTCTACC	20	20	55.0	55.0	57.8	57.8	0.0	0.0	0.388276	109759	110519	898-898	1.000000	77.2
ccsA_p1	ccsA	1	GCGGCATGCGTTTTACTAAA	CCGGGTTCTCTTCAGTTCAC	20	20	45.0	55.0	57.7	58.2	12.4	0.0	0.453323	110706	111665	1149-1149	1.000000	76.5
ccsA_p2	ccsA	2	GCGGCATGCGTTTTACTAAA	TATTGTAGTGATTCGCCGGG	20	20	45.0	50.0	57.7	57.5	12.4	2.2	0.769724	110706	111665	1164-1164	1.000000	74.0
ccsA_p3	ccsA	3	GCGGCATGCGTTTTACTAAA	CGGGTTCTCTTCAGTTCACA	20	20	45.0	50.0	57.7	57.5	12.4	0.0	0.776651	110706	111665	1148-1148	1.000000	73.9
ccsA_p4	ccsA	4	GCGGCATGCGTTTTACTAAA	CCGGCGAATCACTACAGTAT	20	20	45.0	50.0	57.7	57.2	12.4	9.3	1.045523	110706	111665	1181-1181	1.000000	71.8
ccsA_p5	ccsA	5	GCGGCATGCGTTTTACTAAA	AGTATTGTAGTGATTCGCCGG	20	21	45.0	47.6	57.7	57.9	12.4	0.0	1.344065	110706	111665	1166-1166	1.000000	69.4