Hotspot

trnL-UAG-ccsA

SSC · 121375-121509 · 135 bp

Back to Rheum

Pi 0.0249
MarkerSeek score 66.40
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
135
variable_sites
13
pi
0.0249
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TTTCTGGGATGGGGAGTCTT GGTCCGCGAGAGTTATTTGT 50.0 50.0 57.9 58.0 1674-2198 bp 0.947368 84.5
trnL-UAG-ccsA_p2 TTTCTGGGATGGGGAGTCTT GGCGCTGTTATTGCACTTAC 50.0 50.0 57.9 57.8 682-1207 bp 0.947368 84.1
trnL-UAG-ccsA_p3 TTTCTGGGATGGGGAGTCTT TGGGTAAAAATAGCACCGGG 50.0 50.0 57.9 57.9 700-1225 bp 0.842105 79.9
trnL-UAG-ccsA_p4 GATGGGGCTGGTTCCAATAG GGTCCGCGAGAGTTATTTGT 55.0 50.0 58.0 58.0 2196 bp 0.052632 42.0
trnL-UAG-ccsA_p5 GATGGGGCTGGTTCCAATAG GTTCTCGACGAGACTTGCTT 55.0 50.0 58.0 57.9 1951 bp 0.052632 41.6