Hotspot

rbcL-accD

LSC · 58071-58561 · 491 bp

Back to Rheum

Pi 0.0297
MarkerSeek score 72.10
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
491
variable_sites
45
pi
0.0297
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GCTTGTGAAGTGTGGAAGGA ACCTATGTTGGAACTCCTCCT 50.0 47.6 58.0 57.8 591-605 bp 1.0 79.6
rbcL-accD_p2 GCTTGTGAAGTGTGGAAGGA CACCTATGTTGGAACTCCTCC 50.0 52.4 58.0 57.7 592-606 bp 1.0 79.1
rbcL-accD_p3 GCTTGTGAAGTGTGGAAGGA CCACACCTATGTTGGAACTCC 50.0 52.4 58.0 58.3 595-609 bp 1.0 79.0
rbcL-accD_p4 TGCCAAATGGAGTCCTGAAC ACCTATGTTGGAACTCCTCCT 50.0 47.6 58.1 57.8 619-633 bp 1.0 78.7
rbcL-accD_p5 TGCCAAATGGAGTCCTGAAC CACCTATGTTGGAACTCCTCC 50.0 52.4 58.1 57.7 620-634 bp 1.0 78.2