Hotspot

trnT-UGU-trnL-UAA

LSC · 48212-49021 · 810 bp

Back to Rheum

Pi 0.0175
MarkerSeek score 67.20
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
810
variable_sites
61
pi
0.0175
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.96
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 827-1065 bp 1.0 86.2
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC CCGTAGCGTCTACCGATTTC 55.0 55.0 58.2 58.2 858-1096 bp 1.0 85.8
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 821-1059 bp 1.0 85.0
trnT-UGU-trnL-UAA_p4 GAGTCGAACCGATGACCATC CGTCTACCGATTTCGCCATA 55.0 50.0 58.2 57.6 852-1090 bp 1.0 84.6
trnT-UGU-trnL-UAA_p5 CTAACCTCTGAGCTAAGCGG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.5 58.2 819-1057 bp 1.0 83.3