Hotspot

trnT-GGU-psbD

LSC · 33121-34543 · 1423 bp

Back to Rheum

Pi 0.0177
MarkerSeek score 69.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1423
variable_sites
118
pi
0.0177
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.89
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1379-1595 bp 1.0 88.0
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1393-1609 bp 1.0 87.9
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1382-1598 bp 1.0 87.8
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1396-1612 bp 1.0 87.7
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 55.0 50.0 58.1 57.7 1395-1611 bp 1.0 87.5