Hotspot

atpH-atpI

LSC · 14778-15428 · 651 bp

Back to Rheum

Pi 0.0096
MarkerSeek score 70.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
651
variable_sites
35
pi
0.0096
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT CAAGCGGTATTCAGGCTCTT 45.0 50.0 58.0 58.0 592-776 bp 1.0 87.4
atpH-atpI_p2 TACCTTCTACAGCTTGGCCT CAAGCGGTATTCAGGCTCTT 50.0 50.0 58.0 58.0 675-859 bp 1.0 86.9
atpH-atpI_p3 TGGTCCAATAGAAGCAAGCC CAAGCGGTATTCAGGCTCTT 50.0 50.0 57.9 58.0 632-816 bp 1.0 86.4
atpH-atpI_p4 AACGGAAGCGGCAGAAATAA CAAGCGGTATTCAGGCTCTT 45.0 50.0 58.2 58.0 590-774 bp 1.0 86.2
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA CAAGCGGTATTCAGGCTCTT 45.0 50.0 58.2 58.0 593-777 bp 1.0 86.2