Hotspot

trnS-GCU-trnG-UCC

LSC · 9033-10107 · 1075 bp

Back to Rheum

Pi 0.0175
MarkerSeek score 65.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1075
variable_sites
84
pi
0.0175
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.95
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ACGGAAAGAGAGGGATTCGA TACCACTAAACTATACCCGC 50.0 45.0 58.2 54.0 1128-1184 bp 1.0 56.5
trnS-GCU-trnG-UCC_p2 CTCGTACAGCGGATTAGCAA TACCACTAAACTATACCCGC 50.0 45.0 57.8 54.0 1091-1147 bp 1.0 56.3
trnS-GCU-trnG-UCC_p3 GACTCGTACAGCGGATTAGC TACCACTAAACTATACCCGC 55.0 45.0 58.2 54.0 1093-1149 bp 1.0 56.0
trnS-GCU-trnG-UCC_p4 ACGGAAAGAGAGGGATTCGA TTACCACTAAACTATACCCGC 50.0 42.9 58.2 54.9 1129-1185 bp 1.0 55.4
trnS-GCU-trnG-UCC_p5 CTCGTACAGCGGATTAGCAA TTACCACTAAACTATACCCGC 50.0 42.9 57.8 54.9 1092-1148 bp 1.0 55.2