Hotspot

rps16-trnQ-UUG

LSC · 6250-7766 · 1517 bp

Back to Rheum

Pi 0.0202
MarkerSeek score 68.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1517
variable_sites
129
pi
0.0202
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.94
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1430-1633 bp 1.0 85.9
rps16-trnQ-UUG_p2 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1429-1632 bp 1.0 83.8
rps16-trnQ-UUG_p3 GCGTTGCTTTCTACCACATC AGAAATCTAAGTGGGGCGTG 50.0 50.0 57.5 57.6 1489-1692 bp 1.0 83.0
rps16-trnQ-UUG_p4 GCGTTGCTTTCTACCACATC GAAATCTAAGTGGGGCGTGG 50.0 55.0 57.5 58.6 1488-1691 bp 1.0 81.3
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG AGAAATCTAAGTGGGGCGTG 50.0 50.0 57.2 57.6 1488-1691 bp 1.0 80.9