Hotspot

ycf1

SSC · 128654-134311 · 5658 bp

Back to Rhaphiolepis

Pi 0.00169
MarkerSeek score 44.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5658
variable_sites
26
pi
0.00169
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGTCCTCATACCATTCCGGA AAAGACAAAAGAAAGCGCGA 50.0 40.0 57.5 56.9 242 bp 1.0 68.2
ycf1_p2 AGTCCTCATACCATTCCGGA AAGACAAAAGAAAGCGCGAA 50.0 40.0 57.5 56.9 241 bp 1.0 68.2
ycf1_p3 AGTCCTCATACCATTCCGGA AGACAAAAGAAAGCGCGAAA 50.0 40.0 57.5 56.9 240 bp 1.0 68.2
ycf1_p4 AGTCCTCATACCATTCCGGA AAGACAAAAGAAAGCGCGAAA 50.0 38.1 57.5 57.6 241 bp 1.0 65.9
ycf1_p5 AGTCCTCATACCATTCCGGA AGACAAAAGAAAGCGCGAAAA 50.0 38.1 57.5 57.6 240 bp 1.0 65.9