Hotspot

ndhF

SSC · 113915-116170 · 2256 bp

Back to Rhaphiolepis

Pi 0.00117

MarkerSeek score 29.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2256
variable_sites: 7
pi: 0.00117
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	GAACCGGTGACTCAGAAACA	50.0	50.0	58.0	57.8	2549-2554 bp	1.0	78.3
ndhF_p2	TGGGTTGGTATTAGGCTGGA	GAACCGGTGACTCAGAAACA	50.0	50.0	58.0	57.8	2808-2813 bp	1.0	78.3
ndhF_p3	GCAGAATACCGTCACCCATT	GAACCGGTGACTCAGAAACA	50.0	50.0	58.0	57.8	2659-2664 bp	1.0	78.2
ndhF_p4	ACAGGAACAAGAGGGATCCA	GAACCGGTGACTCAGAAACA	50.0	50.0	58.0	57.8	2535-2540 bp	1.0	78.1
ndhF_p5	AAGAGGGATCCACCGAAGAA	GAACCGGTGACTCAGAAACA	50.0	50.0	58.0	57.8	2527-2532 bp	1.0	78.1