Hotspot

ndhA

SSC · 124457-126684 · 2228 bp

Back to Rhaphiolepis

Pi 0.00112

MarkerSeek score 49.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2228
variable_sites: 6
pi: 0.00112
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CTTGTTTGAGACACGTTGTGA	ATTTGCAAATTCTTCCGCGG	42.9	45.0	57.0	58.0	2366-2375 bp	1.0	64.5
ndhA_p2	TCTTGTTTGAGACACGTTGTG	ATTTGCAAATTCTTCCGCGG	42.9	45.0	57.0	58.0	2367-2376 bp	1.0	64.5
ndhA_p3	CTCTTGTTTGAGACACGTTGTG	ATTTGCAAATTCTTCCGCGG	45.5	45.0	58.1	58.0	2368-2377 bp	1.0	64.0
ndhA_p4	TCTCTTGTTTGAGACACGTTGT	ATTTGCAAATTCTTCCGCGG	40.9	45.0	58.1	58.0	2369-2378 bp	1.0	63.4
ndhA_p5	CTTGTTTGAGACACGTTGTGA	GCAAATTCTTCCGCGGTTAG	42.9	50.0	57.0	57.8	2362-2371 bp	1.0	63.1